Rabbit Anti-PMEL Recombinant Antibody (clone P14-V)
CAT#: ZG-0353U
Rabbit anti-PMEL antibody (clone P14-V) displayed high specificity against the PMEL protein of Human.
Tested Data
Gene Expression
IHC-P
Figure 1 Rabbit Anti-PMEL Antibody (ZG-0353U) in IHC-P
Diffuse anti Melanosome antibody positivity in the cutaneous malignant melanoma. Formalin fixed, paraffin embedded human tissue (4 μm section) stained with anti-Melanosome monoclonal antibody.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to endoplasmic reticulum & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/1898_C6_19_cr5ba8a6f6e250d_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_7_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_4_6.jpg
Normal Tissue
Figure 4 Skin
Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185664-PMEL
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Specifications
- Immunogen
- Peptide derived from C-terminal sequence of human melanosome/gp100
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PMEL
- Species Reactivity
- Human
- Clone
- P14-V
- Applications
- IHC-P
- Conjugate
- Unconjugated
- Related Disease
- Vitiligo-Associated Multiple Autoimmune Disease Susceptibility 1 and Melanoma In Congenital Melanocytic Nevus.
Product Property
- Purification
- Affinity Purified
- Buffer
- 20 mM Tris-HCl, pH 8.0, 20 mg/ml BSA and 0.05% sodium azide
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Paraffin. Specific conditions should be optimized by the end user.
IHC-P: 1:100-1:200
Target
- Alternative Names
- P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; HMB-45; ME20-M; PMEL17; D12S53E; melanocyte protein PMEL; melanocyte protein Pmel 17; melanocyte protein mel 17; melanocytes lineage-specific antigen GP100; melanoma-associated ME20 antigen; melanosomal matrix protein17; silver locus protein homolog; silver, mouse, homolog of
- Gene ID
- 6490
- UniProt ID
- P40967
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PMEL"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1280z | Mouse Anti-PMEL Recombinant Antibody (clone 21C9) | ICC, IF, IHC | Mouse IgG1 |
| MOB-0325MZ | Recombinant Mouse Anti-Human PMEL Antibody (clone IMB46) | IHC | Mouse antibody |
| ZG-0592F | Mouse Anti-PMEL Recombinant Antibody (clone HMB45) | IHC-P | Mouse IgG1 |
| VS3-CJ934 | Mouse Anti-PMEL Recombinant Antibody (clone PT1593) | IHC-P | Mouse IgG2b, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G051E | Anti-PMEL immunotoxin (Fab)-PE | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1047CL-F(E) | Anti-Human PMEL Recombinant Antibody Fab Fragment (A3F) | IHC, WB, FC | |
| TAB-1048CL-F(E) | Anti-Human PMEL Recombinant Antibody Fab Fragment (G11B) | IHC, WB, FC | |
| TAB-1049CL-F(E) | Anti-Human PMEL Recombinant Antibody Fab Fragment (J7E) | IHC, WB, FC | |
| TAB-1050CL-F(E) | Mouse Anti-PMEL Recombinant Antibody; Fab Fragment (TAB-1050CL-F(E)) | FC | Mouse Fab |
| TAB-1051CL-F(E) | Mouse Anti-PMEL Recombinant Antibody; Fab Fragment (TAB-1051CL-F(E)) | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2761 | Hi-Affi™ Recombinant Rabbit Anti-PMEL Monoclonal Antibody (DS2761AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0046-LSX-S(P) | Mouse Anti-PMEL Recombinant Antibody; scFv Fragment (HPAB-0046-LSX-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-0047-LSX-S(P) | Mouse Anti-PMEL Recombinant Antibody; scFv Fragment (HPAB-0047-LSX-S(P)) | ELISA, WB, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0046-LSX-F(E) | Mouse Anti-PMEL Recombinant Antibody; Fab Fragment (HPAB-0046-LSX-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-0047-LSX-F(E) | Human Anti-PMEL Recombinant Antibody; Fab Fragment (HPAB-0047-LSX-F(E)) | ELISA, WB, FC | Chimeric Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF186 | H-2Db/HPV16 E7 (RAHYNIVTF) MHC Monomer | MHC Multimer | |
| MHC-YF214 | B*15:01/EBV EBNA4 (GQGGSPTAM) MHC Monomer | MHC Multimer | |
| MHC-YF225 | A*02:01/HPV16 E7 (YMLDLQPET) MHC Monomer | MHC Multimer | |
| MHC-YF295 | A*02:01/HPV E7 (YMLDLQPETT) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC658 | Recombinant Anti-PMEL Vesicular Antibody, EV Displayed (VS-0425-YC658) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5512 | Anti-PMEL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5513 | Anti-Mouse PMEL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5514 | Anti-Rat PMEL Immunohistochemistry Kit | IHC |
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