Rabbit Anti-PMEL Recombinant Antibody (clone R04-2E1) (CAT#: VS3-XY3469)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PMEL

• Species Reactivity
• Human

• Clone
• R04-2E1

• Applications
• WB, IHC-P

• Related Disease
• Vitiligo-Associated Multiple Autoimmune Disease Susceptibility 1; Melanoma In Congenital Melanocytic Nevus

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IHC: 1/50-1/100

• Alternative Names
• P1; SI; SIL; ME20; P100; SILV; HMB45; ME20M; gp100; HMB-45; ME20-M; PMEL17; D12S53E

• Gene ID
• 6490

• UniProt ID
• P40967

• Long Name
• Premelanosome protein

• Sequence Similarities
• Belongs to the PMEL/NMB family.

• Cellular Localization
• Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Secreted

• Post Translation Modifications
• N- and O-glycosylated. A small amount of P1/P100 (major form) undergoes glycosylation in ER and Golgi compartments to yield P2/P120 (minor form). The mature P2 form leaves the trans-Golgi network and is mainly targeted to stage I melanosomes via the plasma membrane and clathrin-mediated endocytosis. Stage II melanosomes harbor only Golgi-modified fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides. O-glycosylation of the RPT region is a conserved feature likely involved in amyloid sheet separation via electrostatic repulsion.

• Protein Refseq
• NP_001186982.1; NP_001186983.1; NP_001307050.1

• Function
• Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody

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