Rabbit Anti-PMEL Recombinant Antibody (clone R04-2E1) (CAT#: VS3-XY3469)
The rabbit recombinant antibody specifically recognizes PMEL (Premelanosome protein), which may prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway. It specifically reacts with human PMEL. It has been tested using Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to endoplasmic reticulum & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/1898_C6_19_cr5ba8a6f6e250d_selected.jpg

(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_7_5.jpg

(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_4_6.jpg

(Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_B_7_1.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185664-PMEL
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PMEL
- Species Reactivity
- Human
- Clone
- R04-2E1
- Applications
- WB, IHC-P
- Related Disease
- Vitiligo-Associated Multiple Autoimmune Disease Susceptibility 1; Melanoma In Congenital Melanocytic Nevus
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- P1; SI; SIL; ME20; P100; SILV; HMB45; ME20M; gp100; HMB-45; ME20-M; PMEL17; D12S53E
- Gene ID
- 6490
- UniProt ID
- P40967
- Long Name
- Premelanosome protein
- Sequence Similarities
- Belongs to the PMEL/NMB family.
- Cellular Localization
- Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Secreted
- Post Translation Modifications
- N- and O-glycosylated. A small amount of P1/P100 (major form) undergoes glycosylation in ER and Golgi compartments to yield P2/P120 (minor form). The mature P2 form leaves the trans-Golgi network and is mainly targeted to stage I melanosomes via the plasma membrane and clathrin-mediated endocytosis. Stage II melanosomes harbor only Golgi-modified fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides. O-glycosylation of the RPT region is a conserved feature likely involved in amyloid sheet separation via electrostatic repulsion.
- Protein Refseq
- NP_001186982.1; NP_001186983.1; NP_001307050.1
- Function
- Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PMEL"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1280z | Mouse Anti-PMEL Recombinant Antibody (clone 21C9) | ICC, IF, IHC | Mouse IgG1 |
HPAB-0046-LSX | Mouse Anti-PMEL Recombinant Antibody (HPAB-0046-LSX) | ELISA, WB, FC, IB, IF | Mouse Fab |
HPAB-0047-LSX | Human Anti-PMEL Recombinant Antibody (HPAB-0047-LSX) | ELISA, WB, FC | Human IgG |
ZG-0592F | Mouse Anti-PMEL Recombinant Antibody (clone HMB45) | IHC-P | Mouse IgG1 |
VS3-CJ934 | Mouse Anti-PMEL Recombinant Antibody (clone PT1593) | IHC-P | Mouse IgG2b, κ |
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