Rabbit Anti-PMEL Recombinant Antibody (clone R04-2E1)
CAT#: VS3-XY3469
The rabbit recombinant antibody specifically recognizes PMEL (Premelanosome protein), which may prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway. It specifically reacts with human PMEL. It has been tested using Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to endoplasmic reticulum & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/1898_C6_19_cr5ba8a6f6e250d_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_7_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_A_4_6.jpg
Normal Tissue
Figure 4 Skin
Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31649/144626_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185664-PMEL
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PMEL
- Species Reactivity
- Human
- Clone
- R04-2E1
- Applications
- WB, IHC-P
- Related Disease
- Vitiligo-Associated Multiple Autoimmune Disease Susceptibility 1; Melanoma In Congenital Melanocytic Nevus
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- P1; SI; SIL; ME20; P100; SILV; HMB45; ME20M; gp100; HMB-45; ME20-M; PMEL17; D12S53E
- Gene ID
- 6490
- UniProt ID
- P40967
- Long Name
- Premelanosome protein
- Sequence Similarities
- Belongs to the PMEL/NMB family.
- Cellular Localization
- Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Secreted
- Post Translation Modifications
- N- and O-glycosylated. A small amount of P1/P100 (major form) undergoes glycosylation in ER and Golgi compartments to yield P2/P120 (minor form). The mature P2 form leaves the trans-Golgi network and is mainly targeted to stage I melanosomes via the plasma membrane and clathrin-mediated endocytosis. Stage II melanosomes harbor only Golgi-modified fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides. O-glycosylation of the RPT region is a conserved feature likely involved in amyloid sheet separation via electrostatic repulsion.
- Protein Refseq
- NP_001186982.1; NP_001186983.1; NP_001307050.1
- Function
- Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PMEL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1280z | Mouse Anti-PMEL Recombinant Antibody (clone 21C9) | ICC, IF, IHC | Mouse IgG1 |
| MOB-0325MZ | Recombinant Mouse Anti-Human PMEL Antibody (clone IMB46) | IHC | Mouse antibody |
| HPAB-0046-LSX | Mouse Anti-PMEL Recombinant Antibody (HPAB-0046-LSX) | ELISA, WB, FC, IB, IF | Mouse Fab |
| HPAB-0047-LSX | Human Anti-PMEL Recombinant Antibody (HPAB-0047-LSX) | ELISA, WB, FC | Human IgG |
| MOB-0212F | Mouse Anti-PMEL Recombinant Antibody (MOB-0212F) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G051E | Anti-PMEL immunotoxin (Fab)-PE | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1047CL-F(E) | Anti-Human PMEL Recombinant Antibody Fab Fragment (A3F) | IHC, WB, FC | |
| TAB-1048CL-F(E) | Anti-Human PMEL Recombinant Antibody Fab Fragment (G11B) | IHC, WB, FC | |
| TAB-1049CL-F(E) | Anti-Human PMEL Recombinant Antibody Fab Fragment (J7E) | IHC, WB, FC | |
| TAB-1050CL-F(E) | Mouse Anti-PMEL Recombinant Antibody; Fab Fragment (TAB-1050CL-F(E)) | FC | Mouse Fab |
| TAB-1051CL-F(E) | Mouse Anti-PMEL Recombinant Antibody; Fab Fragment (TAB-1051CL-F(E)) | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2761 | Hi-Affi™ Recombinant Rabbit Anti-PMEL Monoclonal Antibody (DS2761AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0046-LSX-S(P) | Mouse Anti-PMEL Recombinant Antibody; scFv Fragment (HPAB-0046-LSX-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-0047-LSX-S(P) | Mouse Anti-PMEL Recombinant Antibody; scFv Fragment (HPAB-0047-LSX-S(P)) | ELISA, WB, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0046-LSX-F(E) | Mouse Anti-PMEL Recombinant Antibody; Fab Fragment (HPAB-0046-LSX-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-0047-LSX-F(E) | Human Anti-PMEL Recombinant Antibody; Fab Fragment (HPAB-0047-LSX-F(E)) | ELISA, WB, FC | Chimeric Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF186 | H-2Db/HPV16 E7 (RAHYNIVTF) MHC Monomer | MHC Multimer | |
| MHC-YF214 | B*15:01/EBV EBNA4 (GQGGSPTAM) MHC Monomer | MHC Multimer | |
| MHC-YF225 | A*02:01/HPV16 E7 (YMLDLQPET) MHC Monomer | MHC Multimer | |
| MHC-YF295 | A*02:01/HPV E7 (YMLDLQPETT) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC658 | Recombinant Anti-PMEL Vesicular Antibody, EV Displayed (VS-0425-YC658) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5512 | Anti-PMEL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5513 | Anti-Mouse PMEL Immunohistochemistry Kit | IHC | |
| VS-0525-XY5514 | Anti-Rat PMEL Immunohistochemistry Kit | IHC |
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