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    Mouse Anti-POU5F1 Recombinant Antibody (VS7-0425-WR652) (CAT#: VS7-0425-WR652)

    This product is a mouse IgG antibody that binds to human POU5F1. The antibody can be used for WB, FC, IF, and ICC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line RT4
    Figure 1 IF staining of human cell line RT4
    (Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
    Figure 2 IHC staining of human hippocampus
    Figure 2 IHC staining of human hippocampus
    (Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
    Figure 6 Pancreas
    Figure 6 Pancreas
    (Exocrine glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Pancreatic endocrine cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
    Figure 8 Testis
    Figure 8 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enriched (SuSa)
    Figure 10 RNA cell line category: Cell line enriched (SuSa)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular

    Specifications

    • Immunogen
    • Recombinant human POU5F1 fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human POU5F1
    • Species Reactivity
    • Human
    • Molecule Class
    • Pou Domain, Class 5 Family
    • Applications
    • WB, FC, IF, ICC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
    • Format
    • Liquid
    • Concentration
    • 1 mg/ml
    • Buffer
    • 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
    • Preservative
    • 0.2% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:1000 - 1:2000
      Immunofluorescence: 1:100 - 1:500
      Flow Cytometry: 1:100 - 1:200

    Target

    • Alternative Names
    • POU5F1; OTF4; Oct-4; POU Domain, Class 5, Transcription Factor 1; OCT3; OCT4; POU Class 5 Homeobox 1; Octamer-Binding Protein 4; Octamer-Binding Transcription Factor 3; POU Domain Class 5, Transcription Factor 1; OTF-3; POU-Type Homeodomain-Containing DNA
    • Long Name
    • POU Class 5 Homeobox 1
    • Cellular Localization
    • Cytoplasm; Nucleus
    • Post Translation Modifications
    • Isopeptide bond; Phosphoprotein; Ubl conjugation
    • Function
    • Developmental protein; DNA-binding

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "POU5F1"

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    Please select product type
    IgG Antibody Products Rabbit Monoclonal Antibody Products Cancer Stem Cell Biomarker Specific Antibody Products
    CAT Product Name Application Type
    MOB-3985z Mouse Anti-POU5F1 Recombinant Antibody (clone 43H9) WB, IP, IHC, ELISA Mouse IgG
    MOB-2215MZ Recombinant Mouse Anti-Human POU5F1 Antibody (clone OSH2.2) IHC-P Mouse antibody
    VS4-CJ81 Recombinant Mouse Anti-POU5F1 Antibody (VS4-CJ81) ELISA, WB, ICC, FC Mouse IgG1
    VS-0923-FY125 Recombinant Mouse Anti-POU5F1 Antibody (VS-0923-FY125) ELISA, WB Mouse IgG1
    VS11-QX120 Mouse Anti-POU5F1 Recombinant Antibody (clone 41B1) IHC, WB Mouse IgG2b, κ

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