Mouse Anti-POU5F1 Recombinant Antibody (ZG-0384C)

Name: Mouse Anti-POU5F1 Recombinant Antibody (ZG-0384C)
SKU: ZG-0384C
Rating: 5 (1 reviews)

CAT#: ZG-0384C

This product is a mouse antibody that recognizes POU5F1. It can be used in a wide variety of applications, such as: WB.

Tested Data Gene Expression

Figure 1 Mouse Anti-POU5F1 Antibody (ZG-0384C) in WB

Western blot detection of POU5F1/OCT4 in F9 cell lysates using POU5F1/OCT4 mouse mAb (1:1000 diluted). Predicted band size: 45KDa. Observed band size: 45KDa.

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Figure 2 Mouse Anti-POU5F1 Antibody (ZG-0384C) in WB

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RT4

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human hippocampus

Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg

Normal Tissue Figure 4 Colon

Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg

Normal Tissue Figure 5 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg

Normal Tissue Figure 6 Pancreas

Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg

Normal Tissue Figure 7 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enriched (SuSa)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular

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Specifications

Immunogen
Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E. coli.

Host Species
Mouse

Type
Mouse IgG

Species Reactivity
Mouse

Applications
WB

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
1 mg/mL

Storage
Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
WB 1:1000

Target

Alternative Names
Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7

Gene ID
18999

UniProt ID
P20263

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0384C.pdf

MSDS

MSDS - ZG-0384C.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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