Mouse Anti-PPARG Recombinant Antibody (VS7-0425-WR657) (CAT#: VS7-0425-WR657)
This product is a mouse IgG antibody that binds to human PPARG. The antibody can be used for WB, IF, and ICC assays. It cross reacts with mouse, rat, bovine, dog, goat, pig, rabbit, and sheep.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
Figure 2 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
(Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
Figure 6 Placenta
(Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
(Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
(Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BEWO, CAPAN-2, HSkMC, PC-3, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
Specifications
- Immunogen
- Recombinant human PPARG (C-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPARG
- Species Reactivity
- Human, Mouse, Rat, Bovine, Dog, Goat, Pig, Rabbit, sheep
- Molecule Class
- Peroxisome Proliferator-Activated Receptor (Ppar) Nuclear Receptor
- Applications
- WB, IF, ICC
- Conjugate
- Unconjugated
- Related Disease
- Obesity (OBESITY); Lipodystrophy, familial partial, 3 (FPLD3); Glioma 1 (GLM1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Immunofluorescence: 1:100 - 1:500
Target
- Alternative Names
- PPARG; Peroxisome Proliferator-Activated Nuclear Receptor Gamma Variant 1; GLM1; PPAR-Gamma; PPARG2; CIMT1; Peroxisome Proliferative Activated Receptor, Gamma; Peroxisome Proliferator-Activated Receptor Gamma; PPARG1; NR1C3
- Gene ID
- 5468
- UniProt ID
- P37231
- Long Name
- Peroxisome proliferator activated receptor gamma
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Glycoprotein; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001317544.2
- Function
- Activator; DNA-binding; Receptor
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
mTOR Signaling Pathway
Throid Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1668z | Mouse Anti-PPARG Recombinant Antibody (clone 28C5) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-2265CT | Recombinant Mouse anti-Human PPARG Monoclonal antibody (TQN302) | IHC-P, WB, ELISA, IP, ChIP, GSA | |
| MRO-1258-CN | Recombinant Rabbit Anti-PPARG Monoclonal Antibody (JF101-4) | WB | Rabbit IgG |
| MRO-2129-CN | Rabbit Anti-PPARG Polyclonal Antibody (MRO-2129-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS4-CJ84 | Recombinant Mouse Anti-PPARG Antibody (VS4-CJ84) | ELISA, WB, ICC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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