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    CytoStream™ Rabbit Anti-PRKAA1 Recombinant Antibody (VS13-YC928) (CAT#: VS13-YC928)

    The CytoStream™ Rabbit Anti-PRKAA1 Recombinant Antibody is specific for human and rat PRKAA1. PRKAA1 encodes an AMPK catalytic subunit, regulating cellular energy status by modifying metabolic enzyme activities. The anti-PRKAA1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).

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    • Purity:

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
    Figure 2 IHC staining of human stomach
    Figure 2 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
    Normal Tissue
    Figure 3 Colon
    Figure 3 Colon
    (Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
    Figure 5 Gallbladder
    Figure 5 Gallbladder
    (Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in tubules
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Sertoli cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular

    Specifications

    • Immunogen
    • Recombinant human AMPK alpha, aa 501-550.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human and Rat PRKAA1
    • Species Reactivity
    • Human, Rat
    • Applications
    • WB, ICC, IF, FC, IP
    • Conjugate
    • NONE

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
    • Preservative
    • 0.05% Sodium azide
    • Storage
    • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Shipping with ice pack.

    Applications

    • Application Notes
    • Western blot (WB): 1/1000-1/5000
      Immunocytochemistry (ICC): 1/50-1/200
      Immunofluorescence (IF): 1/50-1/200
      Flow cytometry (FC): 1/50-1/100

    Target

    • Alternative Names
    • AMPK; AMPKa1; AMPK alpha 1
    • Long Name
    • protein kinase AMP-activated catalytic subunit alpha 1
    • Cellular Localization
    • Nucleus; Cytoplasm

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "PRKAA1"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Rabbit Monoclonal Antibody Products IgG Antibody Products Antibody Magnetic Beads IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    MOR-2844 Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (DS2844AB) ELISA, IHC-P, WB IgG
    MOR-4672 Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (TH186DS) WB, IF, ICC IgG
    MOR-0064-FY Rabbit Anti-PRKAA1 Recombinant Antibody (clone AFY0035) ICC, IHC-P, WB Rabbit IgG

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