Mouse Anti-PRKAA1 Recombinant Antibody (clone 5G11)
CAT#: ZG-0029J
This product is a mouse antibody that recognizes PRKAA1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-PRKAA1 Recombinant Antibody (clone 5G11) in WB
Western blot analysis of 1)Hela, 2) 293T, 3)3T3, 4) PC12 with AMPK a1 Mouse mAb diluted at 1:2,000.
IHC
Figure 2 Mouse Anti-PRKAA1 Recombinant Antibody (clone 5G11) in IHC
Immunohistochemical analysis of paraffin-embedded Mouse ColonTissue using AMPK a1 Mouse mAb diluted at 1:200.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular
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Specifications
- Immunogen
- Synthetic Peptide of AMPK α1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PRKAA1
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 5G11
- Applications
- WB, IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
- Alternative Names
- AMPK; AMPKa1; AMPK alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK subunit alpha-1; AMPK, alpha, 1; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1
- Gene ID
- 5562
- UniProt ID
- Q13131
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PRKAA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
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| MOR-2844 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (DS2844AB) | ELISA, IHC-P, WB | IgG |
| MOR-4672 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (TH186DS) | WB, IF, ICC | IgG |
| MOR-0064-FY | Rabbit Anti-PRKAA1 Recombinant Antibody (clone AFY0035) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0089-CN | Recombinant Mouse Anti-PRKAA1 Monoclonal Antibody (B1-E10) | WB, IF, IHC | Mouse IgG1 |
| MRO-0090-CN | Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (CBACN-025) | WB, IF, IP, FC | Rabbit IgG |
| VS3-QX912 | Mouse Anti-PRKAA1 Recombinant Antibody (clone 5C9-5B10-6D3) | WB, IHC | Mouse IgG1 |
| VS3-QX913 | Mouse Anti-PRKAA1 Recombinant Antibody (clone 1H3-1G8-5D8) | WB, IHC | Mouse IgG1 |
| VS3-QX914 | Mouse Anti-PRKAA1 Recombinant Antibody (clone 1E8-E3-A2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY215 | AbPlus™ Anti-PRKAA1 Magnetic Beads (CBACN-025) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC928 | CytoStream™ Rabbit Anti-PRKAA1 Recombinant Antibody (VS13-YC928) | WB, ICC, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1741 | Anti-PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5671 | Anti-Mouse PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5670 | Anti-Human PRKAA1 Immunohistochemistry Kit | IHC |
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