Mouse Anti-PTK2 Monoclonal Antibody (VS7-0425-WR711)
CAT#: VS7-0425-WR711
This product is a mouse IgG antibody that binds to human PTK2. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_6.jpg
Normal Tissue
Figure 8 Breast
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Myoepithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_1_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169398-PTK2/subcellular
❮
❯
Specifications
- Immunogen
- Recombinant human PTK2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PTK2
- Species Reactivity
- Human, Mouse
- Molecule Class
- Non-Receptor Protein Tyrosine Kinase
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Aberrant PTK2/FAK1 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. PTK2/FAK1 overexpression is seen in many types of cancer
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PTK2; Protein Tyrosine Kinase 2; FAK1; PTK2 Protein Tyrosine Kinase 2; Protein-Tyrosine Kinase 2; Protein Phosphatase 1; EC 2.7.10.2; Pp125FAK; FAK-Related Non-Kinase Polypeptide; FADK 1; Focal Adhesion Kinase 1; FAK; EC 2.7.10
- Gene ID
- 5746
- UniProt ID
- Q05397
- Long Name
- Protein tyrosine kinase 2
- Cellular Localization
- Cell junction; Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001186578.1
- Function
- Developmental protein; Kinase; Transferase; Tyrosine-protein kinase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MOR-4573 | Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH85DS) | WB, IF, ICC, FC | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2719 | PE-A*02:01/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM | |
| MHC-LC2720 | PE-A*02:07/Human PTK2 (SLDLASLIL) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0564-CN | Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (CBACN-222) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2325-CN | Recombinant Rabbit Anti-PTK2 (phosphorylated Tyr397) Monoclonal Antibody (CBACN-616) | WB, IF | Rabbit IgG |
| ZG-0100U | Rabbit Anti-PTK2 Recombinant Antibody (clone 3B2) | ELISA, IHC | Rabbit IgG |
| VS3-WK2123 | Rabbit Anti-PTK2 (pY397) Recombinant Antibody (clone R01-3L2) | WB, ICC, IF | Rabbit IgG |
| VS7-0425-WR712 | Mouse Anti-PTK2 Monoclonal Antibody (VS7-0425-WR712) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY219 | AbPlus™ Anti-PTK2 Magnetic Beads (CBACN-222) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC956 | CytoStream™ Rabbit Anti-PTK2 Recombinant Antibody (VS13-YC956) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5845 | Anti-PTK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5846 | Anti-Mouse PTK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5847 | Anti-Rat PTK2 Immunohistochemistry Kit | IHC |
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