Rabbit Anti-RB1 (pS807) Recombinant Antibody (clone R04-3H1)
CAT#: VS3-WK2115
This product is a rabbit recombinant antibody that is specific to phosphorylated RB1. The target of this antibody is phospho-RB1 (Ser807). The clone R04-3H1 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, and IHC staining at a dilution of 1/50-1/100. The molecular weight of RB1 (pSer807) observed in WB experiments using this antibody is 106 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/95/656_B4_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50082/143536_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139687-RB1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser807 of human, mouse and rat RB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3H1
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "RB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1820z | Mouse Anti-RB1 Recombinant Antibody (clone 30C9) | WB, IHC, IP | Mouse IgG1, κ |
| MRO-2370-CN | Recombinant Rabbit Anti-RB1 (phosphorylated Ser807) Monoclonal Antibody (CBACN-656) | WB, IF, IHC | Rabbit IgG |
| ZG-060R | Mouse Anti-RB1 Recombinant Antibody (ZG-060R) | ELISA, IHC | Mouse IgG |
| VS3-XY1360 | Mouse Anti-RB1 Recombinant Antibody (clone 7E4B8) | ELISA, IHC | Mouse IgG1 |
| VS3-FY2861 | Rabbit Anti-RB1 Recombinant Antibody (VS3-FY2861) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3015 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (DS3015AB) | ICC, IF, IP, WB | IgG |
| MOR-4704 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH218DS) | WB, IF, ICC, FC | IgG |
| MOR-4705 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH219DS) | WB, IF, ICC, FC | IgG |
| MOR-4706 | Hi-Affi™ Recombinant Rabbit Anti-RB1 Monoclonal Antibody (TH220DS) | WB, IF, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6011 | Anti-RB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6012 | Anti-Mouse RB1 Immunohistochemistry Kit | IHC |
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