Mouse Anti-RPS6 Recombinant Antibody (VS3-CJ1094)
CAT#: VS3-CJ1094
This product is a mouse antibody that recognizes human, mouse, and rat RPS6 (N-Terminus).
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31153/63143_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31153/63143_A_7_3.jpg
Normal Tissue
Figure 3 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31153/63143_A_1_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31153/63143_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31153/63143_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31153/63143_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (NB-4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137154-RPS6
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Specifications
- Immunogen
- Recombinant protein of RPS6
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse, Rat RPS6 (N-Terminus)
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IF, IHC-P, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000), Immunofluorescence (1:25), Immunohistochemistry-Parraffin (1:25), Flow Cytometry (1:25).
Target
- Alternative Names
- S6
- Gene ID
- 6194
- UniProt ID
- P62753
- Sequence Similarities
- Belongs to the eukaryotic ribosomal protein eS6 family.
- Cellular Localization
- Cytoplasm, Cytoplasmic ribonucleoprotein granule, Cytosol, Cytosolic ribosome
- Post Translation Modifications
- Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the pre-initiation complex.
Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.
Mono-ADP-ribosylation at Glu-35 by PARP16 inhibits polysome assembly and mRNA loading, thereby inhibiting protein translation.
- Protein Refseq
- NP_001001.2
- Function
- Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279).
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Related Diseases
Non-small Cell Lung Cancer
Related Signaling Pathways
Ras Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "RPS6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1951z | Mouse Anti-RPS6 Recombinant Antibody (clone 26A10) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0814MZ | Recombinant Mouse Anti-Human RPS6 Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3120 | Hi-Affi™ Recombinant Rabbit Anti-RPS6 Monoclonal Antibody (DS3120AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4707 | Hi-Affi™ Recombinant Rabbit Anti-RPS6 Monoclonal Antibody (TH221DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4730 | Hi-Affi™ Recombinant Rabbit Anti-RPS6 Monoclonal Antibody (TH244DS) | WB, IF, ICC, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2372 | PE-A*03:01/Human RPS6 (SVLNLVIVK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2373 | PE-A*11:01/Human RPS6 (SVLNLVIVK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0084-FY | Rabbit Anti-RPS6 Recombinant Antibody (clone AFY0055) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX979 | Mouse Anti-RPS6 Recombinant Antibody (clone 7B10-E11-E11) | WB, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX980 | Mouse Anti-RPS6 Recombinant Antibody (clone 981CT2.1.1) | WB, ICC, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1280 | Recombinant Rabbit Anti-RPS6 Antibody (clone R05-3A4) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1967 | Anti-RPS6 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6218 | Anti-Mouse RPS6 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6217 | Anti-Human RPS6 Immunohistochemistry Kit | IHC |
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