Anti-RSPO3 Antibody Prodrug, Protease Activated (VS-1025-YC134)
CAT#: VS-1025-YC134
The Anti-RSPO3 Antibody Prodrug, Protease Activated (VS-1025-YC134) is an antibody prodrug comprised of a recombinant human antibody targeting human RSPO3, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to RSPO3 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to RSPO3 effectively. RSPO3 (R-spondin 3) is a ligand that acts as a powerful agonist of the Wnt signaling pathway. Gene fusions involving RSPO3 are highly specific oncogenic drivers in certain subsets of colorectal cancer. This fusion leads to the massive, aberrant overexpression of RSPO3, resulting in hyperactivation of the Wnt/beta-catenin pathway.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_7_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A549, BJ hTERT+, hTERT-RPE1, U-138 MG, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146374-RSPO3
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human RSPO3
- Target
- RSPO3
- Species Reactivity
- Human, Mouse
- Isotype
- IgG1, kappa
- Affinity Constant
- KD = 0.448 nM
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
- Alternative Names
- RSPO3; R-spondin 3; PWTSR; THSD2; CRISTIN1; R-spondin-3
- Gene ID
- 84870
- UniProt ID
- Q9BXY4
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1025-YC134. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "RSPO3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2146-VHH | Recombinant Anti-Human RSPO3 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-350CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-350CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-351CT | Humanized Anti-RSPO3 Recombinant Antibody (TAB-351CT) | WB, ELISA, Neut, FC, IHC, IP | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-353CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-353CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-354CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-354CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-355CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-355CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-356CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-356CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-351CT-S(P) | Humanized Anti-RSPO3 Recombinant Antibody; scFv Fragment (TAB-351CT-S(P)) | WB, ELISA, Neut, FC, IHC, IP | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-351CT-F(E) | Humanized Anti-RSPO3 Recombinant Antibody; Fab Fragment (TAB-351CT-F(E)) | WB, ELISA, Neut, FC, IHC, IP | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-449CQ | Anti-Human RSPO3 Recombinant Antibody (Rosmantuzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0036CQ | Human Anti-RSPO3 Recombinant Antibody (HPAB-0036CQ) | ELISA, Inhib | Humanized IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0037CQ | Human Anti-RSPO3 Recombinant Antibody (HPAB-0037CQ) | ELISA, Inhib, FC | Humanized IgG2, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038CQ | Mouse Anti-RSPO3 Recombinant Antibody (HPAB-0038CQ) | ELISA, Inhib | Mouse IgG2, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0036CQ-S(P) | Human Anti-RSPO3 Recombinant Antibody scFv Fragment (HPAB-0036CQ-S(P)) | ELISA, Inhib | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0037CQ-S(P) | Human Anti-RSPO3 Recombinant Antibody scFv Fragment (HPAB-0037CQ-S(P)) | ELISA, Inhib | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038CQ-S(P) | Mouse Anti-RSPO3 Recombinant Antibody scFv Fragment (HPAB-0038CQ-S(P)) | ELISA, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0036CQ-F(E) | Human Anti-RSPO3 Recombinant Antibody Fab Fragment (HPAB-0036CQ-F(E)) | ELISA, Inhib | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0037CQ-F(E) | Human Anti-RSPO3 Recombinant Antibody Fab Fragment (HPAB-0037CQ-F(E)) | ELISA, Inhib | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038CQ-F(E) | Mouse Anti-RSPO3 Recombinant Antibody Fab Fragment (HPAB-0038CQ-F(E)) | ELISA, Inhib | Mouse Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0618-YC | Rat Anti-RSPO3 Recombinant Antibody (clone 4A6) | ELISA | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0619-YC | Rat Anti-RSPO3 Recombinant Antibody (clone 11C10) | ELISA | Rat IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0618-YC-S(P) | Rat Anti-RSPO3 Recombinant Antibody (clone 4A6); scFv Fragment | ELISA | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0619-YC-S(P) | Rat Anti-RSPO3 Recombinant Antibody (clone 11C10); scFv Fragment | ELISA | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0618-YC-F(E) | Rat Anti-RSPO3 Recombinant Antibody (clone 4A6); Fab Fragment | ELISA | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0619-YC-F(E) | Rat Anti-RSPO3 Recombinant Antibody (clone 11C10); Fab Fragment | ELISA | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-449CQ | Afuco™ Anti-RSPO3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-449CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY176 | Human Anti-RSPO3 (clone Rosmantuzumab) scFv-Fc Chimera | ELISA, WB, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6253 | Anti-RSPO3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6254 | Anti-Mouse RSPO3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC282 | Recombinant Anti-RSPO3 Eliminating Antibody, pH-Sensitive (VS-0625-YC282) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC297 | SmartAb™ Recombinant Anti-RSPO3 pH-dependent Antibody (VS-0825-YC297) | ELISA, Inhib | Human IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, Neut, FuncS
Application: WB, IF, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
