Human Anti-RSPO3 (clone Rosmantuzumab) scFv-Fc Chimera
CAT#: VS-0425-FY176
The anti-RSPO3 scFv-FC is a monoclonal antibody designed to target the R-spondin 3 (RSPO3) protein. RSPO3 is a member of the R-spondin family, which plays a role in regulating the Wnt/beta-catenin signaling pathway, which is crucial for cell proliferation, differentiation, and apoptosis. The scFv-FC format of this antibody combines a single-chain variable fragment (scFv) that specifically binds to RSPO3 with the human Fc domain, enhancing its stability, half-life, and potential for therapeutic applications. This antibody can be used for research, diagnostic, and therapeutic purposes, particularly in the fields of oncology and regenerative medicine.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_7_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29957/64165_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A549, BJ hTERT+, hTERT-RPE1, U-138 MG, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146374-RSPO3
❮
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Specifications
- Immunogen
- Human RSPO3 as an immunogen.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human RSPO3
- Species Reactivity
- Human
- Clone
- Rosmantuzumab
- Applications
- ELISA, Western Blot, Flow Cytometry
- Related Disease
- Fibrotic disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- R-Spondin 3; Thrombospondin Type-1 Domain-Containing Protein 2; Thrombospondin, Type I, Domain Containing 2; Protein With TSP Type-1 Repeat; Roof Plate-Specific Spondin-3; THSD2; PWTSR
- Gene ID
- 84870
- UniProt ID
- Q9BXY4
- Long Name
- R-Spondin 3
- Sequence Similarities
- Belongs to the R-spondin family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Glycosylation at Asn36
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 4 sites
- Protein Refseq
- XP_016866867.1; XP_054212572.1; NP_116173.2
- Function
- Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis.
Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes.
Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.
Acts as a ligand for frizzled FZD8 and LRP6.
May negatively regulate the TGF-beta pathway.
Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells.
Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2146-VHH | Recombinant Anti-Human RSPO3 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-350CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-350CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| TAB-353CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-353CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| TAB-354CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-354CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| TAB-355CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-355CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| TAB-356CT | Mouse Anti-RSPO3 Recombinant Antibody (TAB-356CT) | WB, ELISA, Neut, FC, IHC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-351CT | Humanized Anti-RSPO3 Recombinant Antibody (TAB-351CT) | WB, ELISA, Neut, FC, IHC, IP | Humanized IgG |
| TAB-351CT-S(P) | Humanized Anti-RSPO3 Recombinant Antibody; scFv Fragment (TAB-351CT-S(P)) | WB, ELISA, Neut, FC, IHC, IP | Humanized scFv |
| TAB-351CT-F(E) | Humanized Anti-RSPO3 Recombinant Antibody; Fab Fragment (TAB-351CT-F(E)) | WB, ELISA, Neut, FC, IHC, IP | Humanized Fab |
| TAB-449CQ | Anti-Human RSPO3 Recombinant Antibody (Rosmantuzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0036CQ-S(P) | Human Anti-RSPO3 Recombinant Antibody scFv Fragment (HPAB-0036CQ-S(P)) | ELISA, Inhib | Humanized scFv |
| HPAB-0037CQ-S(P) | Human Anti-RSPO3 Recombinant Antibody scFv Fragment (HPAB-0037CQ-S(P)) | ELISA, Inhib | Humanized scFv |
| HPAB-0038CQ-S(P) | Mouse Anti-RSPO3 Recombinant Antibody scFv Fragment (HPAB-0038CQ-S(P)) | ELISA, Inhib | Mouse scFv |
| HPAB-M0618-YC-S(P) | Rat Anti-RSPO3 Recombinant Antibody (clone 4A6); scFv Fragment | ELISA | Rat scFv |
| HPAB-M0619-YC-S(P) | Rat Anti-RSPO3 Recombinant Antibody (clone 11C10); scFv Fragment | ELISA | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0036CQ-F(E) | Human Anti-RSPO3 Recombinant Antibody Fab Fragment (HPAB-0036CQ-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-0037CQ-F(E) | Human Anti-RSPO3 Recombinant Antibody Fab Fragment (HPAB-0037CQ-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-0038CQ-F(E) | Mouse Anti-RSPO3 Recombinant Antibody Fab Fragment (HPAB-0038CQ-F(E)) | ELISA, Inhib | Mouse Fab, κ |
| HPAB-M0618-YC-F(E) | Rat Anti-RSPO3 Recombinant Antibody (clone 4A6); Fab Fragment | ELISA | Rat Fab |
| HPAB-0539-FY-F(E) | Mouse Anti-RSPO3 Recombinant Antibody; Fab Fragment (HPAB-0539-FY-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0618-YC | Rat Anti-RSPO3 Recombinant Antibody (clone 4A6) | ELISA | Rat IgG2a |
| HPAB-M0619-YC | Rat Anti-RSPO3 Recombinant Antibody (clone 11C10) | ELISA | Rat IgG2 |
| HPAB-M0620-YC | Rat Anti-RSPO3 Recombinant Antibody (clone 15F3) | ELISA | Rat IgG2 |
| HPAB-M0621-YC | Human Anti-RSPO3 Recombinant Antibody (clone hu4A6.L4H2) | ELISA, FuncS | Human IgG1 |
| HPAB-M0622-YC | Human Anti-RSPO3 Recombinant Antibody (clone hu11C10.L5H2) | ELISA, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-449CQ | Afuco™ Anti-RSPO3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-449CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6253 | Anti-RSPO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6254 | Anti-Mouse RSPO3 Immunohistochemistry Kit | IHC |
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