Mouse Anti-S100A9 Recombinant Antibody (clone 1B6H10)
CAT#: VS7-0425-WR814
This product is a mouse IgG1 antibody that binds to human S100A9. The antibody 1B6H10 recognizes specific amino acid domain of human S100A9 (AA: 1-114). It can be used for IHC and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
Normal Tissue
Figure 3 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
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Specifications
- Immunogen
- Recombinant human S100A9 (AA: 1-114) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human S100A9
- Species Reactivity
- Human
- Clone
- 1B6H10
- Molecule Class
- EF-Hand Calcium-Binding Protein Family
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- S100A9; Migration Inhibitory Factor-Related Protein 14; L1Ag; CFAG; CAGB; Calgranulin B; MRP14; S100 Calcium Binding Protein A9; MIF; Protein S100-A9; LIAG; Leukocyte L1 Complex Heavy Chain; MRP-14; S100 Calcium-Binding Protein A9; S100 Calcium Binding Pr
- Gene ID
- 6280
- UniProt ID
- P06702
- Long Name
- S100 Calcium Binding Protein A9
- Cellular Localization
- Cell membrane; Cytoplasm; Cytoskeleton; Membrane; Secreted
- Post Translation Modifications
- Methylation; Phosphoprotein; S-nitrosylation
- Protein Refseq
- NP_002956.1
- Function
- Antimicrobial; Antioxidant
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-S(P) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); scFv Fragment | WB, ELISA, IHC | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-F(E) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); Fab Fragment | WB, ELISA, IHC | Mouse Fab |
| HPAB-2539LY-F(E) | Mouse Anti-S100A9 Recombinant Antibody; Fab Fragment (HPAB-2539LY-F(E)) | ELISA, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1995 | Anti-S100A9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6317 | Anti-Mouse S100A9 Immunohistochemistry Kit | IHC | |
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| CAT | Product Name | Application | Type |
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