Mouse Anti-SMARCA2 Recombinant Antibody (clone 10F4C9 )

Name: Mouse Anti-SMARCA2 Recombinant Antibody (clone 10F4C9 )
SKU: VS7-0425-WR887
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR887

This product is a mouse IgG2a antibody that binds to human SMARCA2. The antibody 10F4C9 can be used for WB, IHC, ICC, and FC assays.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/371_H2_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/374_H2_1_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/372_H2_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69188_B_7_5.jpg

Normal Tissue Figure 6 Parathyroid gland

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_3_8.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_9_3.jpg

Normal Tissue Figure 8 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_4.jpg

Normal Tissue Figure 9 Kidney

Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_7_5.jpg

Normal Tissue Figure 10 Testis

Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_5_6.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29981/69498_A_8_8.jpg

RNA Expression Figure 12 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000080503-SMARCA2

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Specifications

Host Species
Mouse

Type
Mouse IgG2a

Specificity
Human SMARCA2

Species Reactivity
Human

Clone
10F4C9

Molecule Class
SWI/SNF Family

Applications
WB, IHC, ICC, FC

Conjugate
Unconjugated

Related Disease
Nicolaides-Baraitser syndrome (NCBRS); Blepharophimosis-impaired intellectual development syndrome (BIS); Schizophrenia (SCZD)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Target

Alternative Names
SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 2; ATP-Dependent Helicase SMARCA2; BRG1-Associated Factor 190B; Protein Brahma Homolog; Brahma Homolog; SNF2-Alpha; BAF190B; SNF2L2; HBRM; BRM; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin A2; Global Transcription Activator Homologous Sequence; Probable Global Transcription Activator SNF2L2

Gene ID
6595

UniProt ID
P51531

Long Name
SWI/SNF related BAF chromatin remodeling complex subunit ATPase 2

Cellular Localization
Nucleus

Post Translation Modifications
Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation

Protein Refseq
NP_001276325.1

Function
Activator; DNA-binding; Helicase; Hydrolase

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR887.pdf

MSDS

MSDS - VS7-0425-WR887.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

Reconstitution Buffer

CAT
Product Name

Recommended Dilution Buffer

CAT
Product Name

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