Mouse Anti-SOX2 Monoclonal Antibody (VS7-0425-WR912)
CAT#: VS7-0425-WR912
This product is a mouse IgG antibody that binds to human SOX2. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45725/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62851/1273_B4_7_selected.jpg
Normal Tissue
Figure 3 High expression in cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_9_5.jpg
Normal Tissue
Figure 4 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_6_1.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_A_7_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, HAP1, NTERA-2, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181449-SOX2
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Specifications
- Immunogen
- Recombinant human SOX2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SOX2
- Species Reactivity
- Human
- Molecule Class
- Sox (Sry-Related Hmg-Box) Family
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Microphthalmia, syndromic, 3 (MCOPS3)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SOX2; ANOP3; SRY-Related HMG-Box Gene 2; SRY (Sex Determining Region Y)-Box 2; Transcription Factor SOX-2; Transcription Factor SOX2; MCOPS3
- Gene ID
- 6657
- UniProt ID
- P48431
- Long Name
- SRY-Box 2
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_003097.1
- Function
- Activator; Developmental protein; DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SOX2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2438z | Mouse Anti-SOX2 Recombinant Antibody (clone 44A5) | WB, ELISA, IHC | Mouse IgG2b, κ |
| ZG-108R | Mouse Anti-SOX2 Recombinant Antibody (ZG-108R) | WB | Mouse IgG |
| ZG-109R | Mouse Anti-SOX2 Recombinant Antibody (ZG-109R) | WB, ELISA | Mouse IgG |
| ZG-110R | Mouse Anti-SOX2 Recombinant Antibody (ZG-110R) | WB, ELISA, IHC, IF | Mouse IgG |
| VS3-XY1456 | Mouse Anti-SOX2 Recombinant Antibody (clone 10F10) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3377 | Hi-Affi™ Recombinant Rabbit Anti-SOX2 Monoclonal Antibody (DS3377AB) | ICC, IHC-P, WB, sELISA | IgG |
| MOR-0107-FY | Rabbit Anti-SOX2 Recombinant Antibody (clone AFY0078) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0275 | APC-A*02:01/Human SOX2 (SMYLPGAEV) MHC Tetramer | FCM | |
| MHC-CN0276 | PE-A*02:01/Human SOX2 (SMYLPGAEV) MHC Tetramer | FCM | |
| MHC-CN0303 | APC-A*02:01/Human SOX2 (TLMKKDKYTL) MHC Tetramer | FCM | |
| MHC-CN0304 | PE-A*02:01/Human SOX2 (TLMKKDKYTL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC574 | AbPlus™ Anti-SOX2 Magnetic Beads (VS-0724-YC574) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC90 | Mouse Anti-SOX2 Recombinant Antibody (VS-0924-YC90) - Cancer Stem Cell Marker | IHC, WB, IF | Mouse IgG1 |
| VS-0924-YC91 | Rabbit Anti-SOX2 Antibody (VS-0924-YC91) - Cancer Stem Cell Marker | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY201 | CytoStream™ Mouse Anti-SOX2 Recombinant Antibody (clone 245610) | FC | Mouse IgG2a |
| VS-0225-XY202 | CytoStream™ Mouse Anti-SOX2 Recombinant Antibody (VS-0225-XY202) | FC | Mouse IgG1, kappa |
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