Mouse Anti-SOX9 Recombinant Antibody (VS3-CJ981)
CAT#: VS3-CJ981
This product is a mouse antibody that recognizes human SOX9.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22456/if_selected.jpg
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human SOX9
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human SOX9
- Species Reactivity
- Human
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:1000), Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- CMD1; SRA1; CMPD1; SRXX2; SRXY10
- Gene ID
- 6662
- UniProt ID
- P48436
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylated; acetylation impairs nuclear localization and ability to transactivate expression of target genes. Deacetylated by SIRT1.
Phosphorylation at Ser-64 and Ser-211 by PKA increases transcriptional activity and may help delay chondrocyte maturation downstream of PTHLH/PTHrP signaling. Phosphorylation at either Ser-64 or Ser-211 is required for sumoylation, but phosphorylation is not dependent on sumoylation. Phosphorylated on tyrosine residues; tyrosine dephosphorylation by PTPN11/SHP2 blocks SOX9 phosphorylation by PKA and subsequent SUMOylation.
Ubiquitinated; ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1.
Sumoylated; phosphorylation at either Ser-64 or Ser-211 is required for sumoylation. Sumoylation is induced by BMP signaling pathway.
- Protein Refseq
- NP_000337.1
- Function
- Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed:24038782).
Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6 (PubMed:8640233).
Also binds to some promoter regions (By similarity).
Plays a central role in successive steps of chondrocyte differentiation (By similarity).
Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity).
Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity).
Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity).
Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity).
Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells: when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity).
Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes (By similarity).
Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors (By similarity).
In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells: involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix (By similarity).
Controls epithelial branching during kidney development (By similarity).
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Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SOX9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3882z | Mouse Anti-SOX9 Recombinant Antibody (clone 15C2) | WB, ICC, IF, ELISA | Mouse IgG1 |
| ZG-0122U | Rabbit Anti-SOX9 Recombinant Antibody (clone 5H12) | ELISA, IHC | Rabbit IgG |
| VS3-XY1457 | Mouse Anti-SOX9 Recombinant Antibody (clone 1B11) | ELISA, WB, ICC | Mouse IgG1 |
| VS3-WK1930 | Recombinant Rabbit Anti-SOX9 Capture Antibody (clone R65-8T-3) | ELISA | Rabbit IgG |
| VS3-WK1931 | Recombinant Rabbit Anti-SOX9 Detection Antibody (clone R97-6V-1) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3378 | Hi-Affi™ Recombinant Rabbit Anti-SOX9 Monoclonal Antibody (DS3378AB) | WB, ICC, IF, IP | IgG |
| MOR-0106-FY | Rabbit Anti-SOX9 Recombinant Antibody (clone AFY0077) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1045 | Mouse Anti-SOX9 Recombinant Antibody (clone 334CT39.1.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1099 | CytoStream™ Rabbit Anti-SOX9 Recombinant Antibody (VS13-YC1099) | WB, ICC, IF, IHC-P, IP, FC, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6830 | Anti-SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6831 | Anti-Mouse SOX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6832 | Anti-Rat SOX9 Immunohistochemistry Kit | IHC |
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