Mouse Anti-STAT4 Recombinant Antibody (VS3-CJ64)
CAT#: VS3-CJ64
This product is a mouse antibody that recognizes STAT4.
Gene Expression
Normal Tissue
Figure 1 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1860/6071_A_7_5.jpg
Normal Tissue
Figure 2 Testis
Elongated or late spermatids
Staining: Low
Intensity: Weak
Quantity:>75%
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Pachytene spermatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1860/6071_A_6_6.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (Karpas-707, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138378-STAT4/subcellular
❮
❯
Specifications
- Immunogen
- Recombinant fragment of human STAT4 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human STAT4
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- SLEB11
- Gene ID
- 6775
- UniProt ID
- Q14765
- Sequence Similarities
- Belongs to the transcription factor STAT family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Acetylation at Lys-667 is required for JAK2-mediated phosphorylation and activation of STAT4.
Tyrosine phosphorylated upon IL12 and IFN-alpha activation, but not by IFN-gamma in T-lymphocytes and NK cells (PubMed:8943379).
Serine phosphorylation is required for maximal transcriptional activity but not for DNA binding (PubMed:8943379).
Phosphorylation by MAP2K6 at Ser-721 is required for full transcriptional activity induced by IL12 (PubMed:10961885).
However this serine phosphorylation is not required for cell proliferation although critical for IFN-gamma production (PubMed:12213961).
- Protein Refseq
- NP_001230764.1; NP_003142.1
- Function
- Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response (PubMed:8943379, PubMed:10961885).
Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma (PubMed:12213961, PubMed:35614130).
Participates also in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps (By similarity).
After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated (PubMed:7638186, PubMed:10415122).
Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35 (PubMed:11114383,PubMed:34508746).
Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter (By similarity).
In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation (PubMed:26990433).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-CJ64. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Endometrial Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Throid Cancer
Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "STAT4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1968z | Mouse Anti-STAT4 Recombinant Antibody (clone 6C7) | WB, ICC, IF, IHC | Mouse IgG1 |
| MOB-1955CT | Recombinant Mouse anti-Human STAT4 Monoclonal antibody (EML1950) | ICC, IF, IHC-P, WB | |
| MRO-1440-CN | Recombinant Rabbit Anti-STAT4 Monoclonal Antibody (CBACN-531) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK969 | Rabbit Anti-STAT4 Recombinant Antibody (VS3-WK969) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1400 | Recombinant Rabbit Anti-STAT4 Antibody (clone R02-1C1) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0566MZ | Chicken Anti-STAT4 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3428 | Hi-Affi™ Recombinant Rabbit Anti-STAT4 Monoclonal Antibody (DS3428AB) | WB, IP, IHC | IgG |
| MOR-4742 | Hi-Affi™ Recombinant Rabbit Anti-STAT4 Monoclonal Antibody (TH256DS) | WB, IF, ICC, IHC-P, FC, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2196 | Anti-STAT4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6955 | Anti-Mouse STAT4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6954 | Anti-Human STAT4 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
-3.png)
Application: ELISA, FC, Block, SPR
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF
Application: Neut, FC
Application: Neut, FC, IHC-Fr, IP, BA
Application: ELISA, IHC, FC, IP, IF, BL
Application: Neut, ELISA, WB, SPR, IF, EM
Application: FC
Application: ELISA, Neut, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
