AibGenesis™ Rabbit Anti-TFEB Recombinant Antibody (AibG-Rb-TF788)
CAT#: VS-0426-FY788
Discovered on an AI-powered antibody discovery platform, this Protein A purified antibody targets TFEB for renal carcinoma research with broad species reactivity.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67082/1645_F12_4_cr57b564e2472ae_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (BEWO, Daudi, OE19, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112561-TFEB
❮
❯
Specifications
- Immunogen
- Human
- Host Species
- Rabbit
- Type
- Rabbit IgG, Kappa
- Specificity
- Human TFEB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-TF788
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA, Immunoprecipitation
- Conjugate
- Unconjugated, Biotin and FITC are available.
- Related Disease
- Renal Cell Carcinoma
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Buffer
- PBS, 0.05% Protective protein, 50% Glycerol
- Preservative
- 0.05% Proclin 300
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1:2000-1:10000), Immunohistochemistry (1:200-1:1000), Immunocytochemistry/Immunofluorescence (1:200-1:1000), ELISA (1:5000-1:20000), Immunoprecipitation (1:50-1:200)
Target
- Alternative Names
- BHLHE35
- Gene ID
- 7942
- UniProt ID
- P19484
- Long Name
- Transcription Factor EB
- Sequence Similarities
- Belongs to the MiT/TFE family.
- Cellular Localization
- Cytoplasm, Lysosome, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-211 by MTOR regulates its subcellular location and activity (PubMed:21617040, PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:24081491, PubMed:30120233, PubMed:35662396).
When nutrients are present, phosphorylation by MTOR promotes association with 14-3-3/YWHA adapters and retention in the cytosol (PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:35662396).
Inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:22576015, PubMed:22343943, PubMed:22692423).
Exported from the nucleus in a mTORC1-dependent manner in response to nutrient availability (PubMed:30120233).
Dephosphorylated by phosphatase PPP3CA following Coxsackievirus B3 infection, leading to nuclear translocation (PubMed:33691586).
Alkylated via a non-enzymatic covalent modification (PubMed:35662396).
Itaconate, an anti-inflammatory metabolite generated in response to lipopolysaccharide, alkylates Cys-212, preventing association with 14-3-3/YWHA adapters, thereby promoting nuclear translocation and activity (PubMed:35662396).
Sumoylated; does not affect dimerization with MITF.
(Microbial infection) Cleavage by Coxsackievirus B3 protease 3C after site Gln-60. This non-phosphorylated cleavage product retains its ability to interact with TFEB, TFE3 or MITF and presents impaired transcriptional activity, resulting in disruption of lysosomal functions and increased viral infection.
- Protein Refseq
- NP_001161299.2; NP_001258872.1; NP_001258873.1
- Function
- Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response (PubMed:21617040, PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:30120233, PubMed:31672913, PubMed:35662396).
Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF (PubMed:1748288, PubMed:19556463, PubMed:29146937).
Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation (PubMed:21617040, PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:35662396).
Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity (PubMed:22576015, PubMed:22343943, PubMed:22692423, PubMed:35662396).
Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes (PubMed:19556463, PubMed:22692423).
Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).
Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy (PubMed:21617040, PubMed:22576015, PubMed:23434374, PubMed:27278822).
In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4⁺ T-cells and thymus-dependent humoral immunity (By similarity).
Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer (PubMed:2115126).
Plays a role in the signal transduction processes required for normal vascularization of the placenta (By similarity).
Itaconate-mediated alkylation activates TFEB-dependent lysosomal biogenesis, facilitating the bacteria clearance during the antibacterial innate immune response (PubMed:35662396).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0426-FY788. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TFEB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1543z | Mouse Anti-TFEB Recombinant Antibody (clone 30B6) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1545MZ | Recombinant Mouse Anti-Human TFEB Antibody (clone 420D3b) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ748 | Rabbit Anti-TFEB Recombinant Antibody (VS3-CJ748) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1439 | Recombinant Rabbit Anti-TFEB Antibody (clone R05-0D7) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0923-FY144 | Recombinant Mouse Anti-TFEB Antibody (VS-0923-FY144) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2274 | Anti-TFEB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7191 | Anti-Mouse TFEB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7190 | Anti-Human TFEB Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, SPR, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
