Mouse Anti-TIGIT Recombinant Antibody (clone 1B4) (CAT#: FAMAB-1760CQ)

Figure 1 Anti-TIGIT Abs modulate T cell responses in vivo.

Wild type B6 mice were immunized s.c. with 100 µg MOG35-55 peptide in CFA and received 100 µg anti-TIGIT (or isotype control: Armenian hamster IgG for clone 4D4 or mouse IgG1 for 1G9 and 1B4) antibody i.p on days 0, 2, and 4. On day 10 spleens and lymph nodes were harvested and cells were re-stimulated with MOG35-55 peptide. (dLN: draining lymph node).

Dixon, K. O., Schorer, M., Nevin, J., Etminan, Y., Amoozgar, Z., Kondo, T., ... & Joller, N. (2018). Functional anti-TIGIT antibodies regulate development of autoimmunity and antitumor immunity. The Journal of Immunology, 200(8), 3000-3007.

Figure 2 Functional anti-TIGIT Abs modulate disease severity in EAE.

Wild type B6 mice were immunized s.c. with 100 µg (A, B; D, E) or 10-15µg (C; F) MOG35-55 peptide in CFA, followed by injection of 100 ng pertussis toxin i.v. on day 0 and day 2. Mice also received 100 µg anti-TIGIT (or isotype control: Armenian hamster IgG for clone 4D4 or mouse IgG1 for 1G9 and 1B4) antibody i.p on days 0, 2, 4, 10, and 17 and were monitored daily for EAE.

Dixon, K. O., Schorer, M., Nevin, J., Etminan, Y., Amoozgar, Z., Kondo, T., ... & Joller, N. (2018). Functional anti-TIGIT antibodies regulate development of autoimmunity and antitumor immunity. The Journal of Immunology, 200(8), 3000-3007.

Figure 3 Blocking anti-TIGIT Abs have synergistic effects with blockade of other coinhibitory receptors in cancer.

Wild-type C57BL/6 mice were implanted with MC38 colon carcinoma. Mice with established tumors were treated with anti-PD-1, anti-TIGIT, anti-PD-1 + anti-TIGIT (COMBO), or isotype controls (ISO) and monitored for tumor growth. (A) Mean (left) and individual (right) tumor growth curves are shown (n = 5 per group). Similar results were obtained in an independent experiment. (

Dixon, K. O., Schorer, M., Nevin, J., Etminan, Y., Amoozgar, Z., Kondo, T., ... & Joller, N. (2018). Functional anti-TIGIT antibodies regulate development of autoimmunity and antitumor immunity. The Journal of Immunology, 200(8), 3000-3007.