Mouse Anti-TUBB1 Recombinant Antibody (clone 4D4G6) (CAT#: VS7-0425-WR1068)
This product is a mouse IgG1 antibody that binds to human TUBB1. The antibody 4D4G6 recognizes specific amino acid domain of human TUBB1 (AA: 1-444). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse and monkey.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Normal Tissue
RNA Expression
Figure 1 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4286/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4286/ihc_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_8_5.jpg
Figure 5 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_6_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_6_6.jpg
Figure 6 Fallopian tube
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_7_7.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_7_7.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enriched (HEL)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101162-TUBB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101162-TUBB1
Specifications
- Immunogen
- Recombinant human TUBB1 (AA: 1-444) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TUBB1
- Species Reactivity
- Human, Mouse, Monkey
- Clone
- 4D4G6
- Molecule Class
- Beta Tubulin Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6); Skin creases, congenital symmetric circumferential, 1 (CSCSC1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- si:ch211-122i22.4
- Gene ID
- 203068
- UniProt ID
- P07437
- Long Name
- Tubulin, beta 1 class VI
- Cellular Localization
- Cytoplasm; Cytoskeleton; Microtubule
- Post Translation Modifications
- Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001280141.1
- Function
- Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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MHC Tetramer Products for Other Applications
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1382 | PE-B-FIV21/Gallus gallus TUBB1 (REVDEQLLSV) MHC Tetramer | FCM | |
| MHC-LC1383 | APC-B-FIV21/Gallus gallus TUBB1 (REVDEQLLSV) MHC Tetramer | FCM | |
| MHC-LC1384 | BV421-B-FIV21/Gallus gallus TUBB1 (REVDEQLLSV) MHC Tetramer | FCM |
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