Rabbit Anti-VCL Recombinant Antibody (VS13-YC1213)
CAT#: VS13-YC1213
The Rabbit Anti-VCL Recombinant Antibody is specific for human, mouse and rat VCL. VCL encodes a cytoskeletal protein involved in anchoring F-actin to membranes. Mutations can cause dilated cardiomyopathy. The anti-VCL antibody can be used for Western blot (1/500-1/2000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry (1/200).
Gene Expression
Normal Tissue
Figure 1 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2131/7209_A_6_6.jpg
Normal Tissue
Figure 2 Smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2131/7209_B_4_7.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2131/7209_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2131/7209_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2131/7209_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2131/7209_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, hTERT-RPE1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000035403-VCL
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Specifications
- Immunogen
- Recombinant human Vinculin, aa 1000-1060.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat VCL
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry (IHC): 1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "VCL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3635z | Mouse Anti-VCL Recombinant Antibody (clone 8G3) | WB, IHC, IF, FC, ELISA | Mouse IgG1 |
| MOB-2218CT | Recombinant Mouse anti-Human VCL Monoclonal antibody (TQN338) | ICC, IF, IHC-P, IP, WB | |
| MRO-1586-CN | Rabbit Anti-VCL Recombinant Antibody (clone JM42-43) | WB, IF, IP | Rabbit IgG |
| VS3-FY1540 | Recombinant Rabbit Anti-VCL Antibody (clone R09-1F3) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3831 | Rabbit Anti-VCL Recombinant Antibody (clone DS3831AB) | IHC-P | Rabbit IgG |
| MOR-4759 | Rabbit Anti-VCL Recombinant Antibody (clone TH273DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2455 | PE-A*02:01/Human VCL (GLDVLTAKV) MHC Tetramer | FCM | |
| MHC-LC2456 | PE-A*02:01/Human VCL (RILGAVAKV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1063 | AbPlus™ Anti-VCL Magnetic Beads (VS-0724-YC1063) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7824 | Anti-VCL Immunohistochemistry Kit | IHC | |
| VS-0525-XY7825 | Anti-Mouse VCL Immunohistochemistry Kit | IHC |
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