Rabbit Anti-WT1 Recombinant Antibody (clone 5F2) (CAT#: ZG-0040U)
The purified Rabbit Anti-WT1 Recombinant Antibody (clone 5F2) displayed high specificity against the WT1 protein of human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-WT1 Antibody (ZG-0040U) in WB
Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate. All lanes: Wilms Tumor Protein antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 48, 48, 49, 35, 57, 56, 34 kDa
Observed band size: 50 kDa
IHC
Figure 2 Rabbit Anti-WT1 Antibody (ZG-0040U) in IHC
IHC image of ZG-0040U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Wilms Tumor Protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human WT1
- Species Reactivity
- Human
- Clone
- 5F2
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Wilms Tumor 1 and Frasier Syndrome
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1458z | Mouse Anti-WT1 Recombinant Antibody (clone 24C8) | FC, IF, IHC | Mouse IgG1, κ |
| MOB-0344MZ | Recombinant Mouse Anti-Human Wilms Tumor 1 Antibody (clone 7G-H3) | IHC | Mouse antibody |
| MOB-117CQ | Human Anti-WT1 Recombinant Antibody (MOB-117CQ) | ELISA, WB, FC, FuncS | Human IgG1 |
| ZG-0176C | Mouse Anti-WT1 Recombinant Antibody (clone ABT-WT1) | IHC-P, IF | Mouse IgG |
| ZG-0177C | Mouse Anti-WT1 Recombinant Antibody (clone PT0264) | IHC-P, IF | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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