Chicken Anti-ZIKV E Recombinant Antibody (clone ZIKVEF3S13) (CAT#: VS-0323-CJ70)

• Immunogen
• ZIKV_E recombinant proteins with His-tag

• Host Species
• Chicken

• Type
• Chicken IgY

• Specificity
• Zika virus envelope (ZIKV_E) protein

• Species Reactivity
• ZIKV

• Clone
• ZIKVEF3S13

• Applications
• ELISA, WB

• Conjugate
• Unconjugated

• Related Disease
• Meningoencephalitis; Myelitis; Guillain-Barré syndrome; Microcephaly

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in ELISA, Western Blot.

• Alternative Names
• ZIKV_gp1

• Gene ID
• 7751225

• UniProt ID
• EZIKV

• Sequence Similarities
• In the N-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0-1 NS5-type methyltransferase family.

• Cellular Localization
• Virion; Host nucleus; Host cytoplasm

• Post Translation Modifications
• Envelope protein E:
  N-glycosylation plays a role in virulence in mammalian and mosquito hosts, but may have no effect on neurovirulence.
  Ubiquitination by host TRIM7 promotes virus attachment and fusion of the virus and the host endosome membrane.

• Protein Refseq
• YP_002790881.1

• Function
• Envelope protein E:
  Binds to host cell surface receptors and mediates fusion between viral and cellular membranes. Efficient virus attachment to cell is, at least in part, mediated by host HAVCR1 in a cell-type specific manner (By similarity).
  In addition, host NCAM1 can also be used as entry receptor (By similarity).
  Interaction with host HSPA5 plays an important role in the early stages of infection as well (By similarity).
  Envelope protein is synthesized in the endoplasmic reticulum and forms a heterodimer with protein prM. The heterodimer plays a role in virion budding in the ER, and the newly formed immature particle is covered with 60 spikes composed of heterodimers between precursor prM and envelope protein E. The virion is transported to the Golgi apparatus where the low pH causes the dissociation of PrM-E heterodimers and formation of E homodimers. PrM-E cleavage is inefficient, many virions are only partially matured and immature prM-E proteins could play a role in immune evasion (By similarity).

Isotype Control

• BRD-0674MZ
• Chicken IgY antibodies Isotype

• MOB-191CQ
• Chicken IgY Isotype Control Antibody

Secondary Antibody

• 2ND-006CQ
• Goat Anti-Chicken IgY (H&L) Antibody

• 2ND-007CQ
• Rabbit Anti-Chicken IgY (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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