Why is this specific CD1d tetramer used as a negative control?

This CD1d tetramer is unloaded (contains no ligand). Therefore, it cannot specifically bind to the TCR of NKT cells, making it an ideal negative control to assess non-specific background binding in flow cytometry assays.

Is this control compatible with APC-labeled specific tetramers?

Yes, this negative control is labeled with APC, making it directly comparable to APC-labeled specific CD1d tetramers (e.g., loaded with alpha-GalCer) within the same experimental setup.

APC-Mouse CD1d Tetramer - Negative Control

CAT#: MHC-YF797

This product is a tetramer of CD1d tetramer loaded with no ligand and fluorescent-labeled with APC. It can not bind to mouse NKT cells, as a negative control. Also, this negative control can be used for low frequency positive populations to be accurately quantified.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line HEK 293

Immunofluorescent staining of human cell line HEK 293 shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72662/1466_F8_4_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line HaCaT

Immunofluorescent staining of human cell line HaCaT shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72662/1576_B7_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72662/1521_F8_2_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_B_7_5.jpg

Normal Tissue Figure 5 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_A_7_3.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_A_6_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_A_9_8.jpg

Normal Tissue Figure 8 Spleen

Cells in white pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_B_8_4.jpg

❮

❯

Specifications

Allele
CD1d

Class
Non-classical MHC

MHC Species
Human

Antigen Species
Mouse

Conjugate
APC

Application
FCM

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Breast cancer biomarkers at key points during disease progression

Necessary APC CD1d Control

This unloaded APC-labeled CD1d tetramer is an indispensable negative control for our experiments. It allows us to determine the level of non-specific binding in our mouse NKT cell assays, ensuring the validity of our positive staining results.

Critical For NKT Flow Cytometry

We rely on this negative control reagent to set accurate gates in our flow cytometry analysis. It consistently shows low background, which helps us confirm the specificity of our alpha-GalCer loaded tetramers in the same panel.

Q&As

Why is this specific CD1d tetramer used as a negative control?

A: This CD1d tetramer is unloaded (contains no ligand). Therefore, it cannot specifically bind to the TCR of NKT cells, making it an ideal negative control to assess non-specific background binding in flow cytometry assays.
Is this control compatible with APC-labeled specific tetramers?

A: Yes, this negative control is labeled with APC, making it directly comparable to APC-labeled specific CD1d tetramers (e.g., loaded with alpha-GalCer) within the same experimental setup.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Datasheet

Datasheet - MHC-YF797.pdf

MSDS

MSDS - MHC-YF797.pdf

COA

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