Why is this CD1d tetramer considered a negative control?

This CD1d tetramer is loaded with no ligand (empty). Because it lacks the specific antigen required to engage the NKT cell receptor, it does not bind to mouse NKT cells and serves as a negative control to assess background staining.

What species is this CD1d molecule derived from?

This CD1d molecule is derived from Mouse. It is a non-classical MHC molecule that presents glycolipid antigens, and this specific control product is used in murine NKT cell research.

PE-Mouse CD1d Tetramer - Negative Control

CAT#: MHC-YF794

This product is a tetramer of CD1d tetramer loaded with no ligand and fluorescent-labeled with PE. It can not bind to mouse NKT cells, as a negative control. Also, this negative control can be used for low frequency positive populations to be accurately quantified.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line HEK 293

Immunofluorescent staining of human cell line HEK 293 shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72662/1466_F8_4_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line HaCaT

Immunofluorescent staining of human cell line HaCaT shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72662/1576_B7_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72662/1521_F8_2_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_B_7_5.jpg

Normal Tissue Figure 5 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_A_7_3.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_A_6_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_A_9_8.jpg

Normal Tissue Figure 8 Spleen

Cells in white pulp
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16107/35972_B_8_4.jpg

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Specifications

Allele
CD1d

Class
Non-classical MHC

MHC Species
Human

Antigen Species
Mouse

Conjugate
PE

Application
FCM

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Essential CD1d Negative Control

This unloaded CD1d tetramer is a crucial negative control for our NKT cell research. Labeled with PE, it allows us to accurately determine background staining and validate the specificity of our ligand-loaded tetramers in mouse samples.

Critical For Accurate Gating

We use this negative control reagent in every flow cytometry panel involving CD1d. It ensures that the populations we identify with loaded tetramers are genuine. The product is consistent and behaves exactly as expected for a control reagent.

Q&As

Why is this CD1d tetramer considered a negative control?

A: This CD1d tetramer is loaded with no ligand (empty). Because it lacks the specific antigen required to engage the NKT cell receptor, it does not bind to mouse NKT cells and serves as a negative control to assess background staining.
What species is this CD1d molecule derived from?

A: This CD1d molecule is derived from Mouse. It is a non-classical MHC molecule that presents glycolipid antigens, and this specific control product is used in murine NKT cell research.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Datasheet

Datasheet - MHC-YF794.pdf

MSDS

MSDS - MHC-YF794.pdf

COA

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