CytoStream™ Mouse Anti-CD45.2 Recombinant Antibody (MMC-M1007)
CAT#: VS-0126-XU8
CytoStream™ Mouse Anti-CD45.2 Recombinant Antibody (MMC-M1007) is a high-performance reagent for the unambiguous identification of CD45.2-positive leukocytes. Produced via recombinant platforms, this antibody eliminates the variability found in polyclonal or crude hybridoma supernatants, ensuring absolute specificity for the alloantigen. It is optimized for flow cytometry to provide sharp population separation, which is critical for quantifying low-frequency donor cells in reconstitution assays. This antibody's high purity and defined binding characteristics make it a prerequisite for rigorous hematopoietic research and lineage tracing studies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/if_selected.jpg
Normal Tissue
Figure 2 Appendix
Lymphoid tissue
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_3_2.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, JURKAT, MOLT-4, THP-1, U-266/70, U-266/84, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081237-PTPRC
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| Sub Cat | Product Name | Size | Formulation | Conjugate Group | Characteristics | Stability | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0126-XU8-1 | CytoStream™ Mouse Anti-CD45.2 Recombinant Antibody (MMC-M1007), FITC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | FITC | Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel. | Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh. | ||||||
| VS-0126-XU8-2 | CytoStream™ Mouse Anti-CD45.2 Recombinant Antibody (MMC-M1007), APC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | APC | Protein-based fluorochrome with excitation/emission at ~650/660 nm. Exhibits minimal spectral overlap with FITC and PE, ideal for multicolor panels. High brightness. | Sensitive to light and heat. Store at 4°C in the dark. Avoid repeated freeze-thaw cycles. Stable for ≥1 year under proper storage. | ||||||
| VS-0126-XU8-3 | CytoStream™ Mouse Anti-CD45.2 Recombinant Antibody (MMC-M1007), PE-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy7 | Tandem dye (PE excited, Cy7 emits at ~785 nm). Critical for deep-red infrared detection. Essential: Use a Cy7 (780/60 nm) detector, not a PE filter. Susceptible to tandem bond degradation. | Low stability. Highly sensitive to fixation, light exposure, and storage time. Protect from light and analyze immediately post-fixation. | ||||||
| VS-0126-XU8-4 | CytoStream™ Mouse Anti-CD45.2 Recombinant Antibody (MMC-M1007), Pacific Blue-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Pacific Blue | Synthetic blue fluorescent dye (Ex/Em: ~410/455 nm). Excellent for violet laser (405 nm) excitation. Low spectral overlap with green/red channel fluorophores, simplifying panel design. | High photostability. Tolerates common fixation protocols well. Store at 4°C in the dark for long-term stability. | ||||||
| VS-0126-XU8-5 | CytoStream™ Mouse Anti-CD45.2 Recombinant Antibody (MMC-M1007), PE-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE | Phycobiliprotein fluorochrome (Ex/Em peak: 565/575 nm). One of the brightest fluorophores available, ideal for low-abundance antigen detection. Broad emission requires careful compensation. | Moderate stability. Sensitive to light, heat, and harsh fixatives. Store at 4°C in the dark. Avoid freeze-thaw cycles to maintain performance. |
More Infomation
Specifications
- Host Species
- Mouse
- Type
- Monoclonal Antibody
- Specificity
- CD45.2
- Species Reactivity
- Mouse
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Storage
- Store at 4˚C.
- Shipping
- Ice Packs
Applications
- Application Notes
- Test: 5 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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