Hamster Anti-CXCL12 scFv-Fc Chimera (VS-0425-FY17)
CAT#: VS-0425-FY17
An scFv-Fc fusion of a hamster monoclonal antibody was generated to target CXCL12. This antibody effectively blocks the interaction of CXCL12α with its cognate receptors, CXCR4 and CXCR7, in a dose-responsive manner, and recognizes all three isoforms of the cytokine (α, β, and γ) in both mouse and human.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17564/39207_B_7_5.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17564/39207_A_7_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17564/39207_A_6_6.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17564/39207_B_4_4.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (ASC diff, BJ hTERT+, BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, fHDF/TERT166, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107562-CXCL12
❮
❯
Specifications
- Immunogen
- Recombinant human CXCL12α.
- Host Species
- Hamster
- Type
- Hamster IgG, scFv-Fc
- Specificity
- Human CXCL12
- Species Reactivity
- Human, Mouse
- Applications
- ELISA, Blocking
- Related Disease
- Ovarian cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Blocking: 2 μg/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- C-X-C Motif Chemokine Ligand 12; Pre-B Cell Growth-Stimulating Factor; Chemokine (C-X-C Motif) Ligand 12; Intercrine Reduced In Hepatomas; Stromal Cell-Derived Factor 1; SDF1; PBSF; IRH; C-X-C Motif Chemokine 12
- Gene ID
- 6387
- UniProt ID
- P48061
- Long Name
- C-X-C Motif Chemokine Ligand 12
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively.
The N-terminal processing is probably achieved by DPP4.
Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus.
The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- NP_000600.1; NP_001029058.1; NP_001171605.1; NP_001264919.1; NP_954637.1
- Function
- Chemoattractant active on T-lymphocytes and monocytes but not neutrophils.
Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis.
SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity.
Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites.
Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1.
Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0425-FY17. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CXCL12"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-256-F(E) | Recombinant Anti-human CXCL12 Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| PFBL-057 | Human Anti-CXCL12 Recombinant Antibody; Fab Fragment (PFBL-057) | FC, ELISA, FuncS | Human Fab |
| HPAB-0050LY-F(E) | Human Anti-CXCL12 Recombinant Antibody; Fab Fragment (HPAB-0050LY-F(E)) | Block, ELISA, WB, RIA, FC | Human Fab |
| HPAB-0051LY-F(E) | Human Anti-CXCL12 Recombinant Antibody; Fab Fragment (HPAB-0051LY-F(E)) | Block, ELISA, WB, RIA, FC | Human Fab |
| HPAB-0052LY-F(E) | Human Anti-CXCL12 Recombinant Antibody; Fab Fragment (HPAB-0052LY-F(E)) | Block, ELISA, WB, RIA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-256-S(P) | Recombinant Anti-human CXCL12 Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| PSBL-057 | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (PSBL-057) | FC, ELISA, FuncS | Human scFv |
| HPAB-0050LY-S(P) | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (HPAB-0050LY-S(P)) | Block, ELISA, WB, RIA, FC | Human scFv |
| HPAB-0051LY-S(P) | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (HPAB-0051LY-S(P)) | Block, ELISA, WB, RIA, FC | Human scFv |
| HPAB-0052LY-S(P) | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (HPAB-0052LY-S(P)) | Block, ELISA, WB, RIA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-040 | Recombinant Anti-Human CXCL12 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| HPAB-0348-YC-VHH | Llama Anti-CXCL12 Recombinant Antibody; VHH Fragment (HPAB-0348-YC-VHH) | FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-415CT | Anti-Human CXCL12 Recombinant Antibody (30D8) | Inhib, WB, FC, Funcs, ELISA, Neut | |
| TAB-415CT-S(P) | Anti-Human CXCL12 Recombinant Antibody scFv Fragment (30D8) | WB, ELISA, Neut, FC | |
| TAB-415CT-F(E) | Anti-Human CXCL12 Recombinant Antibody Fab Fragment (30D8) | WB, ELISA, Neut, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-416CT | Anti-Human CXCL12 Recombinant Antibody (hu30D8) | Funcs, FC, PK, ELISA, Neut, WB | Humanized antibody |
| TAB-416CT-S(P) | Anti-Human CXCL12 Recombinant Antibody scFv Fragment (hu30D8) | WB, ELISA, Neut, FC | Humanized antibody |
| TAB-416CT-F(E) | Anti-Human CXCL12 Recombinant Antibody Fab Fragment (hu30D8) | WB, ELISA, Neut, FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-417CT | Human Anti-CXCL12 Recombinant Antibody (TAB-417CT) | BL, WB, ELISA | Human IgG |
| TAB-417CT-S(P) | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (TAB-417CT-S(P)) | BL, WB, ELISA | Human scFv |
| TAB-418CT-S(P) | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (TAB-418CT-S(P)) | BL, WB, ELISA | Human scFv |
| TAB-419CT-S(P) | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (TAB-419CT-S(P)) | BL, WB, ELISA | Human scFv |
| TAB-420CT-S(P) | Human Anti-CXCL12 Recombinant Antibody; scFv Fragment (TAB-420CT-S(P)) | BL, WB, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-612CQ | Mouse Anti-CXCL12 Recombinant Antibody (clone CBL825) | Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0884 | Hi-Affi™ Rabbit Anti-CXCL12 Recombinant Antibody (clone DS884AB) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0050LY | Human Anti-CXCL12 Recombinant Antibody (HPAB-0050LY) | Block, ELISA, WB, RIA, FC | Human IgG1 |
| HPAB-0051LY | Human Anti-CXCL12 Recombinant Antibody (HPAB-0051LY) | Block, ELISA, WB, RIA, FC | Human IgG1 |
| HPAB-0052LY | Human Anti-CXCL12 Recombinant Antibody (HPAB-0052LY) | Block, ELISA, WB, RIA, FC | Human IgG1 |
| MRO-1371-CN | Rabbit Anti-CXCL12 Recombinant Antibody (clone CBACN-498) | WB, IF | Rabbit IgG |
| VS3-CJ980 | Rabbit Anti-CXCL12 Recombinant Antibody (VS3-CJ980) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1813 | Anti-CXCL12 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1814 | Anti-Rat CXCL12 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC82 | Recombinant Anti-CXCL12 Eliminating Antibody, pH-Sensitive (hu30D8 var.) | Antigen-Sweeping In Vivo. | Human IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FuncS
-3.png)
Application: ELISA, FC, Block, SPR
Application: WB, IHC, ELISA, FC
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
Application: ELISA, Block, WB, FC, IP
Application: ELISA, Neut
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
