Human Anti-LOXL2 (clone Simtuzumab) scFv-Fc Chimera
CAT#: VS-0425-FY144
Anti-LOXL2 scFv-FC is a recombinant antibody fragment designed to specifically target lysyl oxidase-like 2 (LOXL2), an enzyme implicated in extracellular matrix remodeling and fibrosis. By inhibiting LOXL2, this scFv-FC can potentially mitigate pathological processes in fibrotic diseases and certain cancers. The fusion to the Fc region enhances stability, half-life, and effector functions, making it a promising candidate for therapeutic applications.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36257/847_B2_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56542/985_A11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate positivity in exocrine cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36257/752_B2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_8_4.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_4_6.jpg
Normal Tissue
Figure 9 Placenta
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_3_7.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+ SV40 Large T+ RasG12V, fHDF/TERT166, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134013-LOXL2
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Specifications
- Host Species
- Human
- Type
- Human IgG4, scFv-Fc
- Specificity
- Human LOXL2
- Species Reactivity
- Human
- Clone
- Simtuzumab
- Applications
- Inhibition
- Related Disease
- Hyperostosis Cranialis Interna and Pelvic Inflammatory Disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- LOR2; LOX L2; LOXL2; Lysyl oxidase homolog 2; Lysyl oxidase like 2; WS9 14
- Gene ID
- 4017
- UniProt ID
- Q9Y4K0
- Long Name
- Lysyl oxidase homolog 2
- Sequence Similarities
- Belongs to the lysyl oxidase family.
- Cellular Localization
- Secreted, extracellular space, extracellular matrix, basement membrane; Nucleus; Chromosome; Endoplasmic reticulum
- Post Translation Modifications
- The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
N-glycosylated.
N-glycosylation on Asn-455 and Asn-644 may be essential for proper folding and secretion; may be composed of a fucosylated carbohydrates attached to a trimannose N-linked glycan core.
Glycosylation at Asn288, Asn455, and Asn644
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect LOXL2_HUMAN (1480)
Glycosylation from GlyGen 4 sites, 16 N-linked glycans (2 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- NP_002309.1
- Function
- Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine).
Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation.
Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2).
Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription.
LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "LOXL2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1260z | Mouse Anti-LOXL2 Recombinant Antibody (clone 19H6) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
| HPAB-0160-LSX | Mouse Anti-LOXL2 Recombinant Antibody (HPAB-0160-LSX) | ELISA | Mouse IgG |
| HPAB-0161-LSX | Human Anti-LOXL2 Recombinant Antibody (HPAB-0161-LSX) | ELISA, WB, RIA | Human IgG |
| HPAB-0162-LSX | Mouse Anti-LOXL2 Recombinant Antibody (HPAB-0162-LSX) | ELISA, WB, RIA | Mouse IgG |
| VS3-FY856 | Recombinant Rabbit Anti-LOXL2 Antibody (clone R09-7J5) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-890 | Anti-Human LOXL2 Recombinant Antibody (Simtuzumab) | Inhib, Agonist | IgG4 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0693CL | Mouse Anti-LOXL2 Recombinant Antibody (TAB-0693CL) | IF, ELISA, FC | Mouse IgG |
| TAB-0693CL-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (TAB-0693CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0693CL-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (TAB-0693CL-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2075 | Hi-Affi™ Recombinant Rabbit Anti-LOXL2 Monoclonal Antibody (DS2075AB) | ICC, IF, FC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0160-LSX-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0160-LSX-S(P)) | ELISA | Mouse scFv |
| HPAB-0161-LSX-S(P) | Human Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0161-LSX-S(P)) | ELISA, WB, RIA | Human scFv |
| HPAB-0162-LSX-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0162-LSX-S(P)) | ELISA, WB, RIA | Mouse scFv |
| HPAB-0163-LSX-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0163-LSX-S(P)) | ELISA, WB, RIA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0160-LSX-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0160-LSX-F(E)) | ELISA | Mouse Fab |
| HPAB-0161-LSX-F(E) | Human Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0161-LSX-F(E)) | ELISA, WB, RIA | Human Fab |
| HPAB-0162-LSX-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0162-LSX-F(E)) | ELISA, WB, RIA | Mouse Fab |
| HPAB-0163-LSX-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0163-LSX-F(E)) | ELISA, WB, RIA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-890 | Afuco™ Anti-LOXL2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-890) | FC, IP, ELISA, Neut, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1256 | Anti-LOXL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4072 | Anti-Mouse LOXL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4071 | Anti-Human LOXL2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4073 | Anti-Rat LOXL2 Immunohistochemistry Kit | IHC |
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