Human Anti-MIF scFv-Fc Chimera (VS-0425-FY149) (CAT#: VS-0425-FY149)
The anti-MIF scFv-FC is a recombinant antibody fragment designed to target macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine implicated in various diseases, including cancer and autoimmune disorders. This scFv is engineered as a fusion protein with an Fc region to increase its stability and therapeutic potential.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
Figure 2 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
Figure 4 Prostate
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEK93)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human MIF
- Species Reactivity
- Human
- Applications
- Flow Cytometry, ELISA
- Related Disease
- Rheumatoid Arthritis, Systemic Juvenile and Systemic-Onset Juvenile Idiopathic Arthritis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Macrophage Migration Inhibitory Factor; Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor); Glycosylation-Inhibiting Factor; Phenylpyruvate Tautomerase; L-Dopachrome Tautomerase; L-Dopachrome Isomerase
- Gene ID
- 4282
- UniProt ID
- P14174
- Long Name
- Macrophage Migration Inhibitory Factor
- Sequence Similarities
- Belongs to the MIF family.
- Cellular Localization
- Secreted; Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys78
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 3 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_002406.1
- Function
- Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens.
The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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ScFv Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1484z | Mouse Anti-MIF Recombinant Antibody (clone 19G7) | WB, ELISA, IHC | Mouse IgG1, κ |
| MOB-1923MZ | Recombinant Mouse Anti-Human MIF Antibody (clone 2A10-4D3) | IHC-P, WB | Mouse antibody |
| HPAB-0104-YC | Human Anti-MIF Recombinant Antibody (HPAB-0104-YC) | ELISA, Activ | Human IgG4 |
| VS3-WK1750 | Recombinant Rabbit Anti-MIF Capture Antibody (clone R02-1N7) | ELISA | Rabbit IgG |
| VS3-WK1751 | Recombinant Rabbit Anti-MIF Detection Antibody (clone R07-1X2) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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