Mouse Anti-CASP3 Recombinant Antibody (clone 4H2B10B2) (CAT#: VS3-XY163)
Clone 4H2B10B2 is a mouse antibody, which reacts with human CASP3 protein. This antibody is suitable for CASP3 detection by ELISA, WB, FC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2013_E2_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2013_E2_2_selected.jpg
Figure 2 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/91/ihc_selected.jpg
(Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/91/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2_C1_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2_C1_1_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/3_C1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/3_C1_1_red_green.jpg
Figure 5 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_9_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_9_3.jpg
Figure 6 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
Specifications
- Immunogen
- Recombinant fragment of human CASP3 (a.a. 29-175) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CASP3
- Species Reactivity
- Human
- Clone
- 4H2B10B2
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).
Target
Related Resources
TNF Signaling Pathway
AGE-RAGE Signaling Pathway in Diabetic Complications
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CASP3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1296z | Mouse Anti-CASP3 Recombinant Antibody (clone 24A6) | WB, IHC, IF, ELISA | Mouse IgG1 |
| MRO-1720-CN | Rabbit Anti-CASP3 Polyclonal Antibody (MRO-1720-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1721-CN | Rabbit Anti-CASP3 Polyclonal Antibody (MRO-1721-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0012U | Rabbit Anti-CASP3 Recombinant Antibody (clone 2B10) | ELISA, WB, IHC | Rabbit IgG |
| ZG-0026U | Rabbit Anti-CASP3 Recombinant Antibody (clone 3H12) | ELISA, WB, IHC | Rabbit IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-037 | Recombinant Anti-Human CASP3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B002(A) | Recombinant Anti-human CASP3 Intrabody [(D-Arg)9] | WB, ICC, FuncS | vhh-(D-Arg)9 |
| IAB-B002(G) | Recombinant Anti-human CASP3 Intrabody [+36 GFP] | ICC, Neut, FuncS | vhh-(+36GFP) |
| IAB-B002(T) | Recombinant Anti-human CASP3 Intrabody [Tat] | ELISA, WB, ICC, FuncS | vhh-Tat |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0475 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone DS475AB) | IF, IHC-Fr, IHC-P, WB | Rabbit IgG |
| MOR-4541 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone TH50DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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