Mouse Anti-CCND1 Recombinant Antibody (clone 3D8) (CAT#: VS3-XY183)
Clone 3D8 is a mouse antibody, which reacts with human CCND1 protein. This antibody is suitable for CCND1 detection by ELISA, WB assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1667_B12_1_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1667_B12_1_selected.jpg
Figure 2 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows strong nuclear positivity in a fraction of basal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/ihc_selected.jpg
(Immunohistochemical staining of human esophagus shows strong nuclear positivity in a fraction of basal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/ihc_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1607_B12_2_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1607_B12_2_red_green.jpg
Figure 4 Liver
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_9_4.jpg
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_9_4.jpg
Figure 5 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_6_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (U-266/70, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110092-CCND1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110092-CCND1
Figure 6 RNA cell line category: Cell line enhanced (U-266/70, WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110092-CCND1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110092-CCND1
Specifications
- Immunogen
- Recombinant fragment of human CCND1 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CCND1
- Species Reactivity
- Human
- Clone
- 3D8
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
- Alternative Names
- Cyclin D1; B-Cell Lymphoma 1 Protein; B-Cell CLL/Lymphoma 1; BCL-1 Oncogene; PRAD1 Oncogene; PRAD1; BCL1; Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1)
- Gene ID
- 595
- UniProt ID
- P24385
Related Resources
Breast Cancer
Hedgehog Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G004R | Anti-Ccnd1 immunotoxin (IgG)-RTA | Cytotoxicity assay, Function study |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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