Mouse Anti-TLR3 scFv-Fc Chimera (VS-0425-FY7) (CAT#: VS-0425-FY7)

• Immunogen
• Recombinant human His-tagged TLR3 extracellular domain recombinant protein.

• Host Species
• Mouse

• Type
• Mouse IgG1, scFv-Fc

• Specificity
• Human TLR3

• Species Reactivity
• Human

• Applications
• ELISA, Flow Cytometry, Immunohistochemistry, Inhibition

• Related Disease
• Autoimmune disease, inflammatory disease

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• The optimal dilution concentration is determined by the experiment.

• Alternative Names
• Toll Like Receptor 3; Toll-Like Receptor 3; CD283 Antigen; CD283; IIAE2

• Gene ID
• 7098

• UniProt ID
• O15455

• Long Name
• Toll Like Receptor 3

• Sequence Similarities
• Belongs to the Toll-like receptor family.

• Cellular Localization
• Endoplasmic reticulum membrane; Single-pass type I membrane protein; Endosome membrane; Early endosome

• Post Translation Modifications
• Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346.
  The cleaved form of TLR3 is the predominant form found in endosomes.
  Ubiquitinated by TRIM3; leading to recognition and sorting of polyubiquitinated TLR3 by the ESCRT complexes.
  Ubiquitinated by ZNRF1 via 'Lys-63'-linked ubiquitin chains; leading to TLR3 lysosomal trafficking and degradation.
  Ubiquitinated by RNF170 at Lys-765 via 'Lys-48'-linked ubiquitin chains; leading to TLR3 proteasomal degradation.
  Glycosylation at Asn291, Asn52, Asn57, Asn70, Asn398, Asn124, Asn413, Asn196, Asn507, Asn247, Asn252, Asn265, Asn275, Asn636, and Asn662
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyGen 17 sites, 33 N-linked glycans (10 sites), 1 O-linked glycan (1 site)

• Protein Refseq
• NP_003256.1

• Function
• Key component of innate and adaptive immunity.
  TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms.
  TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection.
  Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.

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