Rabbit Anti-AMPK alpha 1 (phospho S496) Monoclonal Antibody
CAT#: VS-0126-XL94
This rabbit monoclonal identifies the alpha 1 catalytic subunit of AMPK specifically phosphorylated at Serine 496. It acts as a specialized primary tool for energy sensing research in WB and ICC/IF experimental workflows.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular
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Specifications
- Host Species
- Rabbit
- Specificity
- AMPK alpha 1 (phospho S496)
- Species Reactivity
- Human
- Applications
- WB; ICC/IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Utilizing affinity interactions with Protein A beads allows for the systematic harvesting of this exceptionally purified antibody product.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| VS-0325-XY1741 | Anti-PRKAA1 Immunohistochemistry Kit | IHC |
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| VS-0525-XY5670 | Anti-Human PRKAA1 Immunohistochemistry Kit | IHC |
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| VS-1225-XU23 | Knock-Valid™ Rabbit Anti-AMPK alpha 1 (Phospho-Ser496) Monoclonal Antibody | For research on KD and KO validation. |
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| VS-0126-XL295 | Rabbit Anti-AMPK alpha 1 (phospho S496) Monoclonal Antibody (RCB-P0292) | Phospho Antibodies for Cell Signaling Research |
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