Rabbit Anti-ATF2 (phospho T71) Monoclonal Antibody (RCB-P0227)
CAT#: VS-0126-XL70
Specifically targeting the activated Threonine 71 residue of ATF2, this rabbit monoclonal ensures robust epitope recognition. It is an essential reagent for mapping stress-activated transcription pathways via WB, ICC/IF, and IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2
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Specifications
- Host Species
- Rabbit
- Specificity
- ATF2 (phospho T71)
- Species Reactivity
- Human
- Applications
- WB; ICC/IF; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- This product is isolated through a standard ProA resin capture technique for the purpose of refined antibody concentration.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Target
REVIEWS AND Q&AS
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
MAPK Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ATF2"
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| CAT | Product Name | Application | Type |
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| MOB-0673MZ | Mouse Anti-ATF2 Recombinant Antibody (clone CBLS-122) | ELISA | Mouse IgG2a |
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| MOR-0274 | Hi-Affi™ Rabbit Anti-ATF2 Recombinant Antibody (clone DS274AB) | IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-0130-CN | Rabbit Anti-ATF2 Recombinant Antibody (clone CBACN-047) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-2301-CN | Rabbit Anti-ATF2 Recombinant Antibody (clone CBACN-595) | WB, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| ZG-0315F | Mouse Anti-ATF2 Recombinant Antibody (ZG-0315F) | WB, IHC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0551J | Rabbit Anti-ATF2 Recombinant Antibody (clone 4F2) | ELISA, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY22 | AbPlus™ Anti-ATF2 Magnetic Beads (CBACN-047) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL171 | Rabbit Anti-ATF2 (phospho T71) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL961 | Rabbit Anti-ATF-2 (phospho T69) Antibody | Phospho Antibodies for Cell Signaling Research |
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