Rabbit Anti-p68 RNA Helicase (phospho Y593) Antibody
CAT#: VS-0126-XL542
Rabbit polyclonal antibody directed against phospho-Tyrosine 593 of p68 RNA Helicase. It is utilized in WB, IHC, and IF to study the role of tyrosine phosphorylation in epithelial-mesenchymal transition.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/278_G2_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71154/1949_B4_1_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5868/642_D2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45892_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_9_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20043/45622_A_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108654-DDX5
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Specifications
- Host Species
- Rabbit
- Specificity
- DDX5
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IHC-p; IF/ICC; ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Produced via epitope-specific immunogen affinity chromatography from rabbit antiserum to guarantee the removal of contaminants.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:10000
Not yet tested in other applications.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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