Rabbit Anti-S6K1 (phospho T421/S424) Monoclonal Antibody
CAT#: VS-0126-XL142
Recognizing dual phosphorylation at T421/S424, this rabbit monoclonal provides targeted detection of active S6K1. It is a robust tool for protein synthesis and growth signaling analysis via Western Blot (WB).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
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Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- S6K1 (phospho T421/S424)
- Species Reactivity
- Human Rabbit
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Achieved through a specific biochemical purification workflow, ensuring the resulting antibody meets strict quality standards for research.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
Target
- Alternative Names
- p70S6K; p70 S6 kinase alpha; STK14A; PS6K
- UniProt ID
- P23443
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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