Rabbit Anti-Src (phospho Y529) Monoclonal Antibody
CAT#: VS-0126-XL16
Focused on the inhibitory Tyrosine 529 site of Src, this rabbit antibody provides exceptional binding specificity. Use this reagent as a specialized primary tool for characterizing tyrosine kinase repression in WB, IHC, and ICC/IF.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/850_G12_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows membranous and cytoplasmic positivity in cells in seminferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_B_9_5.jpg
Normal Tissue
Figure 4 Stomach
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_1.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4023/11917_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30875/123970_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, HBEC3-KT, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197122-SRC
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Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- Src (phospho Y529)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IHC; ICC/IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Processed through ProA chromatography for optimal protein concentration and final solution clarity.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Target
- Alternative Names
- Proto-oncogene tyrosine-protein kinase Src; c-Src; p60-Src; SRC1; ASV
- UniProt ID
- P12931
REVIEWS AND Q&AS
CITATIONS
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Related Diseases
Bladder Cancer
Throid Cancer
Endocrine Resistance
Related Signaling Pathways
cAMP Signaling Pathway
ErbB Signaling Pathway
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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