Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5)
CAT#: VS3-FY62
This product is a recombinant rabbit antibody that recognizes human, mouse and rat ALCAM protein. It can detect a band of approximately 65 kDa (observedd molecular weight: 105 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/52_B2_2_selected.jpg
Normal Tissue
Figure 2 Parathyroid gland
High expression in parathyroid gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_1_8_val_selected.jpg
Normal Tissue
Figure 3 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_7_5.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_B_7_5.jpg
Normal Tissue
Figure 5 Stomach
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_6_1.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTERT-HME1, U-138 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170017-ALCAM
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ICC
Figure 1. Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5) in ICC.
Immunocytochemical analysis of CD166 (green) in SKOV-3 using CD166 antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5) in WB.
Western blot analysis of CD166 in rat brain, C6 lysates using CD166 Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human CD166
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ALCAM
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-2E5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 65 kDa; Observed MW: 105 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ALCAM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2226z | Mouse Anti-ALCAM Recombinant Antibody (clone 38A2) | WB, ELISA, IHC | Mouse IgG2a |
| MOB-0457CT | Mouse Anti-ALCAM Recombinant Antibody (clone 4G9C23) | ELISA, WB | Mouse IgG2a |
| MOR-0012-FY | Mouse Anti-ALCAM Recombinant Antibody (clone AZN-L50) | FC, FuncS, IF, IP, WB | Mouse IgG2a |
| ZG-0372F | Mouse Anti-ALCAM Recombinant Antibody (ZG-0372F) | IHC, ELISA | Mouse IgG |
| VS-0524-YC42 | Human Anti-ALCAM Recombinant Antibody (VS-0524-YC42) | FC, ADCC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-031CL | Human Anti-ALCAM Recombinant Antibody (TAB-031CL) | ELISA | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-019CQ | Mouse Anti-ALCAM Recombinant Antibody (clone CBL314) | WB, Block | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0134 | Hi-Affi™ Rabbit Anti-ALCAM Recombinant Antibody (clone DS134AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0273 | APC-H-2Kd/Human CD166 (SLNVSAISI) MHC Tetramer | FCM | |
| MHC-CN0274 | PE-H-2Kd/Human CD166 (SLNVSAISI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0951-FY-F(E) | Human Anti-ALCAM Recombinant Antibody; Fab Fragment (HPAB-0951-FY-F(E)) | IHC | Human Fab |
| HPAB-0952-FY-F(E) | Human Anti-ALCAM Recombinant Antibody; Fab Fragment (HPAB-0952-FY-F(E)) | IHC | Human Fab |
| HPAB-0953-FY-F(E) | Human Anti-ALCAM Recombinant Antibody; Fab Fragment (HPAB-0953-FY-F(E)) | IHC | Human Fab |
| HPAB-0954-FY-F(E) | Human Anti-ALCAM Recombinant Antibody; Fab Fragment (HPAB-0954-FY-F(E)) | IHC | Human Fab |
| HPAB-0955-FY-F(E) | Human Anti-ALCAM Recombinant Antibody; Fab Fragment (HPAB-0955-FY-F(E)) | IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0951-FY-S(P) | Human Anti-ALCAM Recombinant Antibody; scFv Fragment (HPAB-0951-FY-S(P)) | IHC | Human scFv |
| HPAB-0952-FY-S(P) | Human Anti-ALCAM Recombinant Antibody; scFv Fragment (HPAB-0952-FY-S(P)) | IHC | Human scFv |
| HPAB-0953-FY-S(P) | Human Anti-ALCAM Recombinant Antibody; scFv Fragment (HPAB-0953-FY-S(P)) | IHC | Human scFv |
| HPAB-0954-FY-S(P) | Human Anti-ALCAM Recombinant Antibody; scFv Fragment (HPAB-0954-FY-S(P)) | IHC | Human scFv |
| HPAB-0955-FY-S(P) | Human Anti-ALCAM Recombinant Antibody; scFv Fragment (HPAB-0955-FY-S(P)) | IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC794 | AbPlus™ Anti-Alcam Magnetic Beads (VS-0724-YC794) | IP, Protein Purification | |
| VS-0724-YC999 | AbPlus™ Anti-ALCAM Magnetic Beads (VS-0724-YC999) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC19 | Rabbit Anti-ALCAM Antibody (VS-0924-YC19) - Cancer Stem Cell Marker | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC532 | Recombinant Anti-ALCAM Vesicular Antibody, EV Displayed (VS-0425-YC532) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY289 | Anti-ALCAM Immunohistochemistry Kit | IHC | |
| VS-0525-XY290 | Anti-Mouse ALCAM Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC214 | SmartAb™ Recombinant Anti-ALCAM pH-dependent Antibody (Clone EJ212/007-Cl2-5) | ELISA | Human IgG1 |
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