Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5) (CAT#: VS3-FY62)

This product is a recombinant rabbit antibody that recognizes human, mouse and rat ALCAM protein. It can detect a band of approximately 65 kDa (observedd molecular weight: 105 kDa).

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ICC

Figure 1. Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5) in ICC.

Figure 1. Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5) in ICC.

Immunocytochemical analysis of CD166 (green) in SKOV-3 using CD166 antibody and DAPI (blue).

WB

Figure 2. Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5) in WB.

Figure 2. Recombinant Rabbit Anti-ALCAM Antibody (clone R03-2E5) in WB.

Western blot analysis of CD166 in rat brain, C6 lysates using CD166 Antibody.


Specifications

  • Immunogen
  • A synthetic peptide of human CD166
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ALCAM
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R03-2E5
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 65 kDa; Observed MW: 105 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95%
  • Format
  • Liquid
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Activated Leukocyte Cell Adhesion Molecule; CD166 Antigen; MEMD; Activated Leucocyte Cell Adhesion Molecule; CD166

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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