Recombinant Rabbit Anti-ATP2A2 Antibody (clone R09-1G2)
CAT#: VS3-FY117
This product is a recombinant rabbit antibody that recognizes human and rat ATP2A2 protein. It can detect a band of approximately 115 kDa (observedd molecular weight: 115 kDa).
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-ATP2A2 Antibody (clone R09-1G2) in WB.
Western blot analysis of SERCA2 ATPase in HeLa lysate K562 using SERCA2 ATPase Antibody.
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Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62605/140223_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62605/140223_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62605/140223_A_9_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62605/140223_A_6_6.jpg
Normal Tissue
Figure 5 High expression in skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62605/140223_B_3_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174437-ATP2A2
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Specifications
- Immunogen
- A synthetic peptide of human SERCA2 ATPase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ATP2A2
- Species Reactivity
- Human, Rat
- Clone
- R09-1G2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 115 kDa; Observed MW: 115 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 2; Calcium Pump 2; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Slow Twitch Skeletal Muscle Isoform; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2; Endoplasmic Reticulum Class 1/2 Ca(2+) ATPase; SR Ca(2+)-ATPase 2; EC 3.6.3.8; SERCA2; ATP2B
- Gene ID
- 488
- UniProt ID
- P16615
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ATP2A2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2963z | Mouse Anti-ATP2A2 Recombinant Antibody (clone 4C9) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1379-CN | Rabbit Anti-ATP2A2 Recombinant Antibody (clone JM10-20) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY202 | Anti-ATP2A2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY613 | Anti-Mouse ATP2A2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY612 | Anti-Human ATP2A2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY614 | Anti-Canine ATP2A2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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