Recombinant Rabbit Anti-Cd63 Antibody (clone R06-4G3) (CAT#: VS3-FY308)

• Immunogen
• A synthetic peptide of mouse CD63

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Mouse Cd63

• Species Reactivity
• Human, Mouse

• Clone
• R06-4G3

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• MW
• Calculated MW: 26 kDa; Observed MW: 40-70 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• CD63 Molecule; Lysosomal-Associated Membrane Protein 3; Ocular Melanoma-Associated Antigen; CD63 Antigen (Melanoma 1 Antigen); Melanoma-Associated Antigen ME491; Tetraspanin-30; Granulophysin; CD63 Antigen; Tspan-30

• Gene ID
• 967

• UniProt ID
• P08962

• Sequence Similarities
• Belongs to the tetraspanin (TM4SF) family.

• Cellular Localization
• Cell membrane, Endosome, Lysosome, Membrane, Secreted

• Post Translation Modifications
• Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).

• Protein Refseq
• NP_001244318.1; NP_001244319.1; NP_001244320.1

• Function
• Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody