Recombinant Rabbit Anti-CD63 Antibody (clone R08-7C9)
CAT#: VS3-FY310
This product is a recombinant rabbit antibody that recognizes human CD63 protein. It can detect a band of approximately 26 kDa (observedd molecular weight: 26-65 kDa).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73506/1798_B5_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/ihc_selected.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_4_6.jpg
Normal Tissue
Figure 8 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26880_B_6_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135404-CD63
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WB
Figure 1. Recombinant Rabbit Anti-CD63 Antibody (clone R08-7C9) in WB.
Western blot analysis of CD63 in HeLa lysates using CD63 antibody.
IHC
Figure 2. Recombinant Rabbit Anti-CD63 Antibody (clone R08-7C9) in IHC.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using an antibody to CD63. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- A synthetic peptide of human CD63
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD63
- Species Reactivity
- Human
- Clone
- R08-7C9
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 26 kDa; Observed MW: 26-65 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- CD63 Molecule; Lysosomal-Associated Membrane Protein 3; Ocular Melanoma-Associated Antigen; CD63 Antigen (Melanoma 1 Antigen); Melanoma-Associated Antigen ME491; Tetraspanin-30; Granulophysin; CD63 Antigen; Tspan-30
- Gene ID
- 967
- UniProt ID
- P08962
- Sequence Similarities
- Belongs to the tetraspanin (TM4SF) family.
- Cellular Localization
- Cell membrane, Endosome, Lysosome, Membrane, Secreted
- Post Translation Modifications
- Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).
- Protein Refseq
- NP_001244318.1; NP_001244319.1; NP_001244320.1
- Function
- Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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