Recombinant Rabbit Anti-CYCS Antibody (clone R02-9A7)
CAT#: VS3-FY433
This product is a recombinant rabbit antibody that recognizes human, mouse and rat CYCS protein. It can detect a band of approximately 12 kDa (observedd molecular weight: 12 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18597/42408_B_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12177_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Round or early spermatids
Staining: Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_6_6.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12177_B_5_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172115-CYCS
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ICC
Figure 1. Recombinant Rabbit Anti-CYCS Antibody (clone R02-9A7) in ICC.
Immunocytochemical analysis of cytochrome C (green) in K562 using cytochrome C antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-CYCS Antibody (clone R02-9A7) in WB.
Western blot analysis of cytochrome c in K562, rat brain, C6, 3T3, Hela lysates using cytochrome c antibody.
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Specifications
- Immunogen
- A synthetic peptide of human Cytochrome C
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CYCS
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-9A7
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 12 kDa; Observed MW: 12 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CYCS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-258-F(E) | Recombinant Anti-human CYCS Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| MHH-258-F(E) | Recombinant Human Anti-human CYCS Antibody Fab Fragment | WB, IF, FuncS | Fab |
| PFBC-073 | Mouse Anti-CYCS Recombinant Antibody (clone E8); Fab Fragment | WB, FuncS | Mouse Fab |
| FAMAB-0243JF-F(E) | Mouse Anti-CYCS Recombinant Antibody (clone 7E4); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0253JF-F(E) | Mouse Anti-CYCS Recombinant Antibody (clone 1G1.E9); Fab Fragment | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-258-S(P) | Recombinant Anti-human CYCS Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| MHH-258-S(P) | Recombinant Human Anti-human CYCS Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| FAMAB-0243JF-S(P) | Mouse Anti-CYCS Recombinant Antibody (clone 7E4); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0253JF-S(P) | Mouse Anti-CYCS Recombinant Antibody (clone 1G1.E9); scFv Fragment | ELISA, FuncS | Mouse scFv |
| FAMAB-0254JF-S(P) | Mouse Anti-CYCS Recombinant Antibody (clone 7D4.H4); scFv Fragment | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0006MZ | Recombinant Chicken Anti-Cytochrome c IgY (1E4) | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-698CQ | Mouse Anti-CYCS Recombinant Antibody (clone 6H2.B4) | IP, Neut | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0895 | Hi-Affi™ Rabbit Anti-CYCS Recombinant Antibody (clone DS895AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1802-CN | Rabbit Anti-CYCS Polyclonal Antibody (MRO-1802-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0221J | Mouse Anti-CYCS Recombinant Antibody (clone 4B10) | WB, IF, ICC, IHC-P | Mouse IgG |
| VS3-WK828 | Mouse Anti-CYCS Recombinant Antibody (VS3-WK828) | WB, IHC | Mouse IgG |
| VS7-HM799 | Mouse Anti-CYCS Recombinant Antibody (clone 5E6B11) | WB, IHC, FCM | Mouse IgG1 |
| VS7-HM800 | Mouse Anti-CYCS Recombinant Antibody (clone 2E4F8) | IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY75 | AbPlus™ Anti-CYCS Magnetic Beads (CBACN-171) | IP, Protein Purification | |
| VS-0724-YC322 | AbPlus™ Anti-CYCS Magnetic Beads (VS-0724-YC322) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY601 | Anti-CYCS Immunohistochemistry Kit | IHC | |
| VS-0525-XY1839 | Anti-Mouse CYCS Immunohistochemistry Kit | IHC | |
| VS-0525-XY1838 | Anti-Human CYCS Immunohistochemistry Kit | IHC | |
| VS-0525-XY1840 | Anti-Canine CYCS Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC40 | Recombinant Anti-CYCS (AA 44-47 x AA 60-62) Biparatopic Antibody, Tandem scFv (Clone 1G1.E9 x Clone 1F5.D1) | ELISA | Tandem scFv |
| VS-0525-YC42 | Recombinant Anti-CYCS (AA 44-47 x AA 60-62) Biparatopic Antibody, Tandem scFv (Clone 2F8.C10 x Clone 6A7.E5) | ELISA | Tandem scFv |
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