Recombinant Rabbit Anti-CYP17A1 Antibody (clone R08-2J5)
CAT#: VS3-FY435
This product is a recombinant rabbit antibody that recognizes human, mouse and rat CYP17A1 protein. It can detect a band of approximately 57 kDa (observedd molecular weight: 57 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48533/2018_B9_3_selected.jpg
Normal Tissue
Figure 2 Low expression in rectum
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48533/142450_A_5_3_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48533/142450_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48533/142450_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48533/142450_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148795-CYP17A1
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WB
Figure 1. Recombinant Rabbit Anti-CYP17A1 Antibody (clone R08-2J5) in WB.
Western blot analysis of cytochrome P450 17A1/CYP17A1 in K562, C6, 3T3 lysates using Cytochrome P450 17A1/CYP17A1 Antibody.
ICC
Figure 2. Recombinant Rabbit Anti-CYP17A1 Antibody (clone R08-2J5) in ICC.
Immunocytochemical analysis of cytochrome P450 17A1 (green) in HeLa cells using cytochrome P450 17A1 antibody and DAPI (blue).
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Specifications
- Immunogen
- A synthetic peptide of human Cytochrome P450 17A1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CYP17A1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-2J5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 57 kDa; Observed MW: 57 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cytochrome P450 Family 17 Subfamily A Member 1; Steroid 17-Alpha-Monooxygenase; Cytochrome P450, Subfamily XVII (Steroid 17-Alpha-Hydroxylase), Adrenal Hyperplasia; Cytochrome P450, Family 17, Subfamily A, Polypeptide 1; 17-Alpha-Hydroxyprogesterone Aldolase; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; CYPXVII; CYP17
- Gene ID
- 1586
- UniProt ID
- P05093
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CYP17A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0898 | Hi-Affi™ Rabbit Anti-CYP17A1 Recombinant Antibody (clone DS898AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0429-CN | Rabbit Anti-CYP17A1 Recombinant Antibody (clone JB93-32) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0458F | Mouse Anti-CYP17A1 Recombinant Antibody (ZG-0458F) | WB | Mouse IgG |
| ZG-0035U | Rabbit Anti-CYP17A1 Recombinant Antibody (clone 5A9) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-QX311 | Mouse Anti-CYP17A1 Recombinant Antibody (clone 6E1-H9-F6) | WB | Mouse IgG2b |
| VS4-WK136 | Mouse Anti-CYP17A1 Recombinant Antibody (VS4-WK136) | ELISA, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY603 | Anti-CYP17A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1846 | Anti-Mouse CYP17A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1845 | Anti-Human CYP17A1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC280 | CytoStream™ Rabbit Anti-CYP17A1 Recombinant Antibody (VS13-YC280) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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