Recombinant Rabbit Anti-EIF4E Antibody (clone R04-5A4) (CAT#: VS3-FY490)

• Immunogen
• A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat EIF4E

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R04-5A4

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 25 kDa; Observed MW: 25 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Eukaryotic Translation Initiation Factor 4E; MRNA Cap-Binding Protein; EIF-4F 25 KDa Subunit; EIF4EL1; EIF-4E; EIF4F

• Gene ID
• 1977

• UniProt ID
• P06730

• Sequence Similarities
• Belongs to the eukaryotic initiation factor 4E family.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

• Protein Refseq
• NP_001124150.1; NP_001124151.1; NP_001317946.1

• Function
• Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).
  In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285).
  May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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