Recombinant Rabbit Anti-GFAP Antibody (clone R05-9H9)
CAT#: VS3-FY588
This product is a recombinant rabbit antibody that recognizes GFAP. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-9H9 is specific for human, rat GFAP. The antigen is human gfap synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
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WB
Figure 1. Recombinant Rabbit Anti-GFAP Antibody (clone R05-9H9) in WB.
Western Blot analysis of GFAP in rat brain, HeLa lysates using GFAP Antibody.
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Specifications
- Immunogen
- Human GFAP Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat GFAP
- Species Reactivity
- Human, Rat
- Clone
- R05-9H9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Glial Fibrillary Acidic Protein; Intermediate Filament Protein; ALXDRD
- Gene ID
- 2670
- UniProt ID
- P14136
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament
- Post Translation Modifications
- Phosphorylated by PKN1.
- Protein Refseq
- NP_001124491.1; NP_001229305.1; NP_002046.1
- Function
- GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-068 | Recombinant Anti-Mouse Gfap VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-083CQ | Llama Anti-GFAP Recombinant Antibody (TAB-083CQ) | IHC, WB, IF | Llama VHH |
| TAB-084CQ | Llama Anti-GFAP Recombinant Antibody (TAB-084CQ) | WB | Llama VHH |
| HPAB-1158-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1158-FY) | WB, ELISA, IHC | Llama VHH |
| HPAB-1159-FY | Recombinant Anti-GFAP Single Domain Antibody (HPAB-1159-FY) | WB, ELISA, IHC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2053MZ | Recombinant Mouse Anti-Human GFAP Antibody (clone 7B7) | ELISA, ICC, IF, IHC-P, WB | Mouse antibody |
| HPAB-0197-WJ | Mouse Anti-GFAP Recombinant Antibody (clone 5C8) | ELISA, IHC | Mouse IgG1 |
| ZG-0074C | Mouse Anti-GFAP Recombinant Antibody (clone ABT-GFAP) | IHC-P, IF | Mouse IgG |
| VS3-WK1285 | Mouse Anti-GFAP Recombinant Antibody (VS3-WK1285) | WB, IHC | Mouse IgG |
| VS7-HM1001 | Mouse Anti-GFAP Recombinant Antibody (clone CBL319HM) | WB, IHC-p, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0805MZ | Chicken Anti-GFAP Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1122MZ | Chicken Anti-GFAP Polyclonal IgY | IHC, IF, WB | Chicken antibody |
| BRD-1174MZ | Chicken Anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal IgY | WB, ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1426 | Hi-Affi™ Rabbit Anti-GFAP Recombinant Antibody (clone DS1426AB) | ICC, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
| MOR-0100-FY | Rabbit Anti-GFAP Recombinant Antibody (clone AFY0071) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-WJ-S(P) | Mouse Anti-GFAP Recombinant Antibody (clone 5C8); scFv Fragment | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0197-WJ-F(E) | Mouse Anti-GFAP Recombinant Antibody (clone 5C8); Fab Fragment | ELISA, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC176 | AbPlus™ Anti-GFAP Magnetic Beads (VS-0724-YC176) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY912 | Anti-GFAP Immunohistochemistry Kit | IHC | |
| VS-0525-XY2784 | Anti-Mouse GFAP Immunohistochemistry Kit | IHC | |
| VS-0525-XY2783 | Anti-Human GFAP Immunohistochemistry Kit | IHC | |
| VS-0525-XY2785 | Anti-Porcine GFAP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC444 | CytoStream™ Mouse Anti-GFAP Recombinant Antibody (VS13-YC444) | WB, IHC-P, ICC, IF, FC | Mouse IgG1 |
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