Recombinant Rabbit Anti-GPX1 Antibody (clone R06-0H1)
CAT#: VS3-FY612
This product is a recombinant rabbit antibody that recognizes GPX1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R06-0H1 is specific for human, rat GPX1. The antigen is synthetic peptide of human glutathione peroxidase 1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of germinal center and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_8_8_selected.jpg
Normal Tissue
Figure 3 IHC staining of human lung
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11582/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44758/124588_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000233276-GPX1
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WB
Figure 1. Recombinant Rabbit Anti-GPX1 Antibody (clone R06-0H1) in WB.
Western Blot analysis of glutathione peroxidase 1 in A549, HL-60 lysates using glutathione peroxidase 1 antibody.
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Specifications
- Immunogen
- Synthetic peptide of human glutathione peroxidase 1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat GPX1
- Species Reactivity
- Human, Rat
- Clone
- R06-0H1
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 22 kDa; Observed MW: 22 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Glutathione Peroxidase 1; Cellular Glutathione Peroxidase; EC 1.11.1.9; GSHPx-1; EC 1.11.1; GSHPX1; GPx-1; GPXD
- Gene ID
- 2876
- UniProt ID
- P07203
- Sequence Similarities
- Belongs to the glutathione peroxidase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
- Protein Refseq
- NP_000572.2
- Function
- Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism (PubMed:11115402).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1497 | Rabbit Anti-GPX1 Recombinant Antibody (clone DS1497AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-0676-CN | Rabbit Anti-GPX1 Recombinant Antibody (clone CBACN-247) | WB, IP | Rabbit IgG |
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| MRO-1897-CN | Rabbit Anti-GPX1 Polyclonal Antibody (MRO-1897-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY114 | AbPlus™ Anti-GPX1 Magnetic Beads (JF0944) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS13-YC463 | CytoStream™ Mouse Anti-GPX1 Recombinant Antibody (VS13-YC463) | WB, IHC-P, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2898 | Anti-GPX1 Immunohistochemistry Kit | IHC |
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