Recombinant Rabbit Anti-GYS1 (phospho Ser641) Antibody (clone R03-3A1) (CAT#: VS3-FY634)
This product is a recombinant rabbit antibody that recognizes GYS1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R03-3A1 is specific for human, mouse, rat GYS1. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser641 of human glycogen synthase 1/gys1.
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WB
Figure 1. Recombinant Rabbit Anti-GYS1 (phospho Ser641) Antibody (clone R03-3A1) in WB.
Western Blot analysis of glycogen synthase 1/GYS1 (Phospho-Ser641) in C6, 3T3 lysates using Phospho-Glycogen Synthase (Ser641) Antibody.
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser641 of human glycogen synthase 1/GYS1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat GYS1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-3A1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 84 kDa; Observed MW: 84 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Glycogen Synthase 1; Glycogen Synthase 1 (Muscle); EC 2.4.1.11; GYS; Glycogen [Starch] Synthase, Muscle; GSY
- Gene ID
- 2997
- UniProt ID
- P13807
- Sequence Similarities
- Belongs to the glycosyltransferase 3 family.
- Cellular Localization
- Cytoplasm, Cytosol
- Post Translation Modifications
- Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity).
Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity).
Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
- Protein Refseq
- NP_001155059.1; NP_002094.2
- Function
- Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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FC Reagents & Kits
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1550 | Rabbit Anti-GYS1 Recombinant Antibody (clone DS1550AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
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