Recombinant Rabbit Anti-GYS1 (phospho Ser641) Antibody (clone R03-3A1)
CAT#: VS3-FY634
This product is a recombinant rabbit antibody that recognizes GYS1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R03-3A1 is specific for human, mouse, rat GYS1. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser641 of human glycogen synthase 1/gys1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41598/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41598/91527_B_1_6_selected.jpg
Normal Tissue
Figure 3 Skeletal muscle.
High expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/ihc_selected.jpg
Normal Tissue
Figure 4 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_B_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_8_8.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104812-GYS1
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WB
Figure 1. Recombinant Rabbit Anti-GYS1 (phospho Ser641) Antibody (clone R03-3A1) in WB.
Western Blot analysis of glycogen synthase 1/GYS1 (Phospho-Ser641) in C6, 3T3 lysates using Phospho-Glycogen Synthase (Ser641) Antibody.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser641 of human glycogen synthase 1/GYS1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat GYS1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-3A1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 84 kDa; Observed MW: 84 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Glycogen Synthase 1; Glycogen Synthase 1 (Muscle); EC 2.4.1.11; GYS; Glycogen [Starch] Synthase, Muscle; GSY
- Gene ID
- 2997
- UniProt ID
- P13807
- Sequence Similarities
- Belongs to the glycosyltransferase 3 family.
- Cellular Localization
- Cytoplasm, Cytosol
- Post Translation Modifications
- Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity).
Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity).
Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
- Protein Refseq
- NP_001155059.1; NP_002094.2
- Function
- Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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