Recombinant Rabbit Anti-LCK Antibody (clone R01-8A5)
CAT#: VS3-FY843
This product is a recombinant rabbit antibody that recognizes LCK. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-8A5 is specific for human, rat LCK . The antigen is human lck recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/if_selected.jpg
Normal Tissue
Figure 2 High expression in lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_8_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3494/11345_A_4_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182866-LCK
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ICC
Figure 1. Recombinant Rabbit Anti-LCK Antibody (clone R01-8A5) in ICC.
Immunocytochemical analysis of Lck (green) in Jurkat using Lck antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-LCK Antibody (clone R01-8A5) in WB.
Western Blot analysis of Lck in Jurkat lysates using Lck antibody.
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Specifications
- Immunogen
- Human Lck recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat LCK
- Species Reactivity
- Human, Rat
- Clone
- R01-8A5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 58 kDa; Observed MW: 58 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- LCK Proto-Oncogene, Src Family Tyrosine Kinase; Lymphocyte Cell-Specific Protein-Tyrosine Kinase; T Cell-Specific Protein-Tyrosine Kinase; Leukocyte C-Terminal Src Kinase; LSK; T-Lymphocyte Specific Protein Tyrosine Kinase P56lck; Lymphocyte-Specific Protein Tyrosine Kinase; Proto-Oncogene Tyrosine-Protein Kinase LCK; P56(LSTRA) Protein-Tyrosine Kinase; Tyrosine-Protein Kinase Lck;Proto-Oncogene Lck; Protein YT16; EC 2.7.10.2; Pp58lck; P56-LCK; P56lck; IMD22; YT16
- Gene ID
- 3932
- UniProt ID
- P06239
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Related Signaling Pathways
TCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LCK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1072CT | Recombinant Mouse anti-Human LCK Monoclonal antibody (9F6G0) | ELISA, WB | |
| VS3-QX666 | Mouse Anti-LCK Recombinant Antibody (clone 845CT3.5.2) | WB | Mouse IgG1 |
| VS3-QX667 | Mouse Anti-LCK Recombinant Antibody (clone 2E5-H5-C9) | WB, IHC, ICC, FC, ELISA | Mouse IgG2a |
| VS3-CJ434 | Mouse Anti-LCK Recombinant Antibody (VS3-CJ434) | WB, ICC, IHC, FC | Mouse IgG |
| VS7-0425-WR280 | Mouse Anti-Lck Monoclonal Antibody (clone 8E5F9) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2023 | Hi-Affi™ Recombinant Rabbit Anti-LCK Monoclonal Antibody (DS2023AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3959 | Anti-LCK Immunohistochemistry Kit | IHC |
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