Recombinant Rabbit Anti-MPO Antibody (clone R03-6F2)
CAT#: VS3-FY943
This product is a recombinant rabbit antibody that recognizes MPO. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R03-6F2 is specific for mouse, rat MPO. The antigen is human myeloperoxidase recombinant protein.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1487_B11_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1403_F7_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1398_F7_1_red_green.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/145726_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21147/146573_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HL-60, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
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Specifications
- Immunogen
- human myeloperoxidase recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse, Rat MPO
- Species Reactivity
- Mouse, Rat
- Clone
- R03-6F2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 84 kDa; Observed MW: 60 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MPO"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1015 | Recombinant Mouse Anti-Human MPO Antibody Fab Fragment | LMNX, ELISA | Fab |
| FAMAB-0256WJ-F(E) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2273 | Hi-Affi™ Recombinant Rabbit Anti-MPO Monoclonal Antibody (DS2273AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0256WJ-S(P) | Human Anti-MPO Recombinant Antibody (clone E3-MPO); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0622WJ | Mouse Anti-MPO Recombinant Antibody (clone 6D6) | ELISA, WB, IF | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0623WJ | Mouse Anti-MPO Recombinant Antibody (clone 9B5) | ELISA, WB, IF | Mouse IgM, κ |
| ZG-0288C | Mouse Anti-MPO Recombinant Antibody (ZG-0288C) | WB, ELISA | Mouse IgG |
| ZG-0525F | Mouse Anti-MPO Recombinant Antibody (clone 8E6) | FC | Mouse IgG1 |
| ZG-0529F | Mouse Anti-MPO Antibody (clone 8E6 and RFB4) | FC | Mouse IgG1 |
| ZG-0530F | Mouse Anti-MPO Antibody (clone 8E6 and UCHT1) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY164 | CytoStream™ Mouse Anti-MPO Recombinant Antibody (clone 8F4/MPO) | FC | Mouse IgG1, kappa |
| VS13-YC738 | CytoStream™ Mouse Anti-MPO Recombinant Antibody (VS13-YC738) | WB, IHC-P, ICC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1365 | Anti-MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4482 | Anti-Mouse MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4481 | Anti-Human MPO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4483 | Anti-Rat MPO Immunohistochemistry Kit | IHC |
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