Recombinant Rabbit Anti-NBN Antibody (clone R04-7C7)
CAT#: VS3-FY1003
This product is a recombinant rabbit antibody that recognizes NBN. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R04-7C7 is specific for human NBN. The antigen is synthetic peptide of human p95 nbs1.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_4_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_5.jpg
Normal Tissue
Figure 7 Cervix
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_7_3.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104320-NBN
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-NBN Antibody (clone R04-7C7) in WB.
Western Blot analysis of p95/NBS1 in HeLa lysates using Nibrin antibody.
ICC
Figure 2. Recombinant Rabbit Anti-NBN Antibody (clone R04-7C7) in ICC.
Immunocytochemical analysis of p95/NBS1 (green) in MCF-7 using p95/NBS1 antibody and DAPI (blue).
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human p95 NBS1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NBN
- Species Reactivity
- Human
- Clone
- R04-7C7
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 85 kDa; Observed MW: 95 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1003. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NBN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-692 | Recombinant Anti-human NBN Antibody | WB, IHC, FuncS | IgG |
| MHH-692 | Recombinant Human Anti-human NBN Antibody | FC, WB, FuncS | IgG |
| MRO-1159-CN | Recombinant Rabbit Anti-NBN Monoclonal Antibody (CBACN-422) | WB, IF, IP, FC, IHC, ChIP | Rabbit IgG |
| VS3-XY1135 | Mouse Anti-NBN Recombinant Antibody (clone 7E4A2) | ELISA, WB, IHC, FC, ICC | Mouse IgG2a |
| VS3-XY1136 | Mouse Anti-NBN Recombinant Antibody (clone 7E4C2) | ELISA, WB, IHC, FC, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-692-F(E) | Recombinant Anti-human NBN Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| MHH-692-F(E) | Recombinant Human Anti-human NBN Antibody Fab Fragment | IF, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-692-S(P) | Recombinant Anti-human NBN Antibody scFv Fragment | WB, IF, FuncS | scFv |
| MHH-692-S(P) | Recombinant Human Anti-human NBN Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2371 | Hi-Affi™ Recombinant Rabbit Anti-NBN Monoclonal Antibody (DS2371AB) | ICC, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY198 | AbPlus™ Anti-NBN Magnetic Beads (CBACN-422) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4696 | Anti-NBN Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, WB, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, WB, FC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
